Fujii Takashi, Wakaizumi Masanori, Ikami Takao, Saito Morio
Institute for Health Sciences, MIKI Corporation, 12-4, Naruohama 3, Nishinomiya 663-8142, Hyogo, Japan.
J Ethnopharmacol. 2008 Sep 2;119(1):53-7. doi: 10.1016/j.jep.2008.05.039. Epub 2008 Jun 6.
Emblica officinalis Gaertn., commonly known as amla, is a rich dietary source of vitamin C, minerals and amino acids, and also contains various phenolic compounds. Amla extract is also known to exhibits potent antioxidant properties and to provide protection for human dermal fibroblasts against oxidative stress, and therefore it is thought to be useful for natural skin care. In this study, we investigated the effects of amla extract on human skin fibroblasts, especially for production of procollagen and matrix metalloproteinases (MMPs), in vitro.
Mitochondrial activity of human skin fibroblasts were measured by WST-8 assay. Quantification of procollagen, MMPs, and Tissue inhibitor of metalloproteinase-1 (TIMP-1) released from human skin fibroblasts were performed by immunoassay technique.
Amla extract stimulated proliferation of fibroblasts in a concentration-dependent manner, and also induced production of procollagen in a concentration- and time-dependent manner. Conversely, MMP-1 production from fibroblasts was dramatically decreased, but there was no evident effect on MMP-2. TIMP-1 was significantly increased by amla extract. From these results, it appears that amla extract works effectively in mitigative, therapeutic and cosmetic applications through control of collagen metabolism.
余甘子，俗称印度醋栗，是维生素C、矿物质和氨基酸的丰富膳食来源，还含有多种酚类化合物。印度醋栗提取物也具有强大的抗氧化特性，能保护人类皮肤成纤维细胞免受氧化应激，因此被认为对天然皮肤护理有益。在本研究中，我们在体外研究了印度醋栗提取物对人类皮肤成纤维细胞的影响，特别是对原胶原蛋白和基质金属蛋白酶（MMPs）产生的影响。
采用WST-8法检测人类皮肤成纤维细胞的线粒体活性。通过免疫测定技术对人类皮肤成纤维细胞释放的原胶原蛋白、MMPs和金属蛋白酶组织抑制剂-1（TIMP-1）进行定量分析。
印度醋栗提取物以浓度依赖的方式刺激成纤维细胞增殖，并以浓度和时间依赖的方式诱导原胶原蛋白的产生。相反，成纤维细胞产生的MMP-1显著减少，但对MMP-2没有明显影响。印度醋栗提取物使TIMP-1显著增加。从这些结果来看，印度醋栗提取物似乎通过控制胶原蛋白代谢在缓解、治疗和美容应用中发挥有效作用。
