Roh Seong Woon, Quan Zhe-Xue, Nam Young-Do, Chang Ho-Won, Kim Kyoung-Ho, Kim Myung-Kyum, Im Wan-Taek, Jin Long, Kim Seung-Hye, Lee Sung-Taik, Bae Jin-Woo
Korea University of Science and Technology, Daejeon 305-333, Republic of Korea.
Int J Syst Evol Microbiol. 2008 Jul;58(Pt 7):1640-3. doi: 10.1099/ijs.0.65783-0.
A Gram-negative strain, PB92(T), which belongs to the family Sphingobacteriaceae, was isolated from soil (Daejeon, Korea). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain PB92(T) was associated with the genus Pedobacter and was most closely related to the type strains Pedobacter sandarakinus DS-27(T) (97.7 %), Pedobacter roseus CL-GP80(T) (97.5 %) and Pedobacter suwonensis 15-52(T) (97.5 %). The major cellular fatty acid components of strain PB92(T) were C(16 : 1)omega7c (21.4 %), iso-C(15 : 0) (30.8 %), iso-C(17 : 0) 3-OH (9.3 %) and iso-C(15 : 0) 2-OH (11.2 %). The G+C content of the genomic DNA from strain PB92(T) was 41.4 mol%. Analysis of 16S rRNA gene sequences, as well as physiological and biochemical tests, indicated that strain PB92(T) could be differentiated genotypically and phenotypically from reference species of the genus Pedobacter. Strain PB92(T) (=KCTC 12511(T)=DSM 19486(T)) is proposed as the type strain of a novel species, Pedobacter agri sp. nov.
从韩国大田的土壤中分离出一株革兰氏阴性菌株PB92(T),它属于鞘氨醇杆菌科。基于16S rRNA基因序列的系统发育分析表明,菌株PB92(T)与Pedobacter属相关,与模式菌株沙红Pedobacter sandarakinus DS-27(T)(97.7%)、玫瑰Pedobacter roseus CL-GP80(T)(97.5%)和水原Pedobacter suwonensis 15-52(T)(97.5%)关系最为密切。菌株PB92(T)的主要细胞脂肪酸成分是C(16 : 1)ω7c(21.4%)、异-C(15 : 0)(30.8%)、异-C(17 : 0) 3-OH(9.3%)和异-C(15 : 0) 2-OH(11.2%)。菌株PB92(T)基因组DNA的G+C含量为41.4 mol%。16S rRNA基因序列分析以及生理生化测试表明,菌株PB92(T)在基因型和表型上可与Pedobacter属的参考物种区分开来。菌株PB92(T)(=KCTC 12511(T)=DSM 19486(T))被提议作为一个新物种——农业Pedobacter agri sp. nov.的模式菌株。