Mohanty Ipseeta Ray, Arya Dharamvir Singh, Gupta Suresh Kumar
Department of Pharmacology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi 29, India.
Clin Nutr. 2008 Aug;27(4):635-42. doi: 10.1016/j.clnu.2008.05.006. Epub 2008 Jul 11.
BACKGROUND & AIMS: The present study was undertaken to evaluate the cardioprotective mechanisms of Withania somnifera (Ws), in the setting of ischemia and reperfusion (IR) injury.
Wistar rats were divided into three groups and received orally saline (sham, control IR) and Ws-50 mg/kg (Ws-IR), respectively, for 1 month. On the 31st day, in the rats of control IR and Ws-IR group, LAD coronary artery occlusion was undertaken for 45 min followed by 1 h reperfusion. Subsequently, all the animals were sacrificed for biochemical, immunohistochemical {Bax and Bcl-2 protein}, terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling (TUNEL) positivity and histopathological studies.
Post-ischemic reperfusion injury resulted in significant cardiac necrosis, apoptosis, decline in antioxidant status and elevation in lipid peroxidation in the IR control group as compared to sham. Ws prior-treatment favorably restored the myocardial oxidant-antioxidant balance, exerted marked anti-apoptotic effects {upregulated Bcl-2 (p<0.001) protein, decreased Bax (p<0.01) protein, and attenuated TUNEL positivity (p<0.01)}, and reduced myocardial damage as evidenced by histopathologic evaluation.
The antioxidant and anti-apoptotic properties of Ws may contribute to the cardioprotective effects.
本研究旨在评估睡茄（Ws）在缺血再灌注（IR）损伤情况下的心脏保护机制。
将Wistar大鼠分为三组，分别口服生理盐水（假手术组、对照IR组）和Ws-50mg/kg（Ws-IR组），持续1个月。在第31天，对对照IR组和Ws-IR组的大鼠进行左冠状动脉前降支闭塞45分钟，随后再灌注1小时。随后，处死所有动物进行生化、免疫组织化学（Bax和Bcl-2蛋白）、末端脱氧核苷酸转移酶生物素-dUTP缺口末端标记（TUNEL）阳性检测和组织病理学研究。
与假手术组相比，缺血再灌注损伤导致IR对照组出现明显的心肌坏死、凋亡、抗氧化状态下降和脂质过氧化升高。Ws预处理有利地恢复了心肌氧化-抗氧化平衡，发挥了显著的抗凋亡作用（上调Bcl-2（p<0.001）蛋白，降低Bax（p<0.01）蛋白，并减弱TUNEL阳性（p<0.01）），并通过组织病理学评估证明减少了心肌损伤。
Ws的抗氧化和抗凋亡特性可能有助于其心脏保护作用。
