Oldenburg Amy L, Crecea Vasilica, Rinne Stephanie A, Boppart Stephen A
Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Opt Express. 2008 Jul 21;16(15):11525-39.
Magnetic nanoparticles (MNPs) are increasingly important in magnetic resonance and biomedical optical imaging. We describe a method for imaging MNPs by detecting nanoscale displacements using a phase-resolved spectral-domain optical coherence tomography (OCT) system. Biological tissues and phantoms are exposed to approximately 800 G magnetic fields modulated at 56 and 100 Hz to mechanically actuate embedded iron oxide MNPs (approximately 20 nm diameter). Sensitivity to 27 microg/g (approximately 2 nM) MNPs within tissue phantoms is achieved by filtering paramagnetic from diamagnetic vibrations. We demonstrate biological feasibility by imaging topically applied MNPs during their diffusion into an excised rat tumor over a 2 hour time period.
磁性纳米颗粒（MNPs）在磁共振和生物医学光学成像中越来越重要。我们描述了一种通过使用相分辨光谱域光学相干断层扫描（OCT）系统检测纳米级位移来对MNPs进行成像的方法。将生物组织和体模暴露于以56和100Hz调制的约800G磁场中，以机械驱动嵌入的氧化铁MNPs（直径约20nm）。通过从抗磁性振动中过滤顺磁性，实现了对组织体模中27μg/g（约2nM）MNPs的灵敏度。我们通过在2小时的时间段内对局部应用的MNPs扩散到切除的大鼠肿瘤中的过程进行成像，证明了其生物学可行性。
