Escudero Raquel, Toledo A, Gil Horacio, Kovácsová Katarina, Rodríguez-Vargas Manuela, Jado Isabel, García-Amil Cristina, Lobo Bruno, Bhide Mangesh, Anda Pedro
Laboratorio de Espiroquetas y Patógenos Especiales, Servicio de Bacteriología, Centro Nacional de Microbiología, Majadahonda, Madrid, Spain.
J Clin Microbiol. 2008 Sep;46(9):3139-43. doi: 10.1128/JCM.00275-08. Epub 2008 Jul 23.
Environmental studies on the distribution of Francisella spp. are hampered by the frequency of Francisella-like endosymbionts that can produce a misleading positive result. A new, efficient molecular method for detection of Francisella tularensis and its discrimination from Francisella-like endosymbionts, as well as two variants associated with human disease (unusual F. tularensis strain FnSp1 and F. tularensis subsp. novicida-like strain 3523), is described. The method is highly specific and sensitive, detecting up to one plasmid copy or 10 genome equivalents.
弗朗西斯菌属分布的环境研究受到类弗朗西斯菌内共生菌频繁出现的阻碍,这些内共生菌可能产生误导性的阳性结果。本文描述了一种新的、高效的分子方法,用于检测土拉弗朗西斯菌并将其与类弗朗西斯菌内共生菌区分开来,以及检测与人类疾病相关的两个变种(不寻常的土拉弗朗西斯菌菌株FnSp1和土拉弗朗西斯菌新凶手亚种样菌株3523)。该方法具有高度特异性和敏感性,可检测至一个质粒拷贝或10个基因组当量。