Zhang Qin, Chang Zai, Yang Jie, Wang Qiang
Department of Chinese Materia Medica Analysis, China Pharmaceutical University, Nanjing, P. R. China 210038.
Phytother Res. 2008 Aug;22(8):1040-5. doi: 10.1002/ptr.2426.
This study investigated the antiatherogenic activity and the chemical constituents of a triterpenoids-enriched extract from the aerial parts of Salvia miltiorrhiza Bunge. The extract displayed a significant, dose-responsive antiatherogenic effect on LDLR(-/-) mice after a 24-week application in a daily dose 50, 100, 200, 1000 mg/kg, respectively. Pronounced systemic inflammation and cutaneous xanthomatosis inhibition effects of the extract, combined with hypocholesteremic and plaque stable properties, were shown in this study. At 200 mg/kg in the high dose treated group, the percent inhibitions of two inflammatory markers in serum, CRP and MCP-1, were 47.6 +/- 4.2% and 36.8 +/- 5.0%, and significant reductions in aortic atherosclerotic lesion areas were 62.3 +/- 3.9% (en face) and 77.8 +/- 3.1% (cross section) compared with the model group. No abnormal behavior or lethality were observed with the extract up to 1000 mg/kg. Fifteen triterpenoids were separated and identified from the extract. This study provided new evidence of the antiatherogenic property of S. miltiorrhiza correlated with triterpenoids by an antiinflammatory mechanism.
本研究考察了丹参地上部分富含三萜类化合物提取物的抗动脉粥样硬化活性及其化学成分。在分别以每日剂量50、100、200、1000 mg/kg给LDLR(-/-)小鼠连续灌胃24周后，该提取物呈现出显著的、剂量依赖性的抗动脉粥样硬化作用。本研究显示，该提取物具有明显的全身炎症抑制作用和皮肤黄色瘤抑制作用，并具有降胆固醇和稳定斑块的特性。在高剂量治疗组（200 mg/kg）中，血清中两种炎症标志物CRP和MCP-1的抑制率分别为47.6±4.2%和36.8±5.0%，与模型组相比，主动脉粥样硬化病变面积显著减少，其中正面观察减少62.3±3.9%，横截面观察减少77.8±3.1%。给予高达1000 mg/kg的提取物未观察到异常行为或致死现象。从该提取物中分离并鉴定出15种三萜类化合物。本研究通过抗炎机制为丹参与三萜类化合物相关的抗动脉粥样硬化特性提供了新证据。
