Yang Fan, Qi Yingxue, Liu Wei, Li Jia, Wang Daijie, Fang Lei, Zhang Yongqing
Key laboratory of Natural Pharmaceutical Chemistry, Shandong University of Traditional Chinese Medicine, Jinan 250200, China.
Key Laboratory of TCM Quality Control Technology, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China.
Molecules. 2019 Sep 23;24(19):3448. doi: 10.3390/molecules24193448.
The aerial parts of Bunge, as the non-medicinal parts, are always discarded during harvesting, resulting in a huge waste of resources and environmental pressure. Due to the high flavonoid content and their antioxidant activities characteristics, the aerial parts of can be developed into natural antioxidants and used in foods. A high-speed counter-current chromatography (HSCCC) method, using a two-phase solvent system composed of -butyl methyl ether/-butanol/acetonitrile/water (3:1:1:20, /), was the first to successfully isolate five flavonoids from the aerial parts of in one attempt, and separately categorized as rutin (), isoquercitrin (), kaempferol-3--α-l-rhamnopyranosyl-(1→6)-β-d-glucopyranoside (), kaempferol-3--β-d-glucopyranoside () and apigenin-7--β-d-glucopyranoside () after identification. The purities of these plant isolates were 97.3%, 99.5%, 92.8%, 98.1% and 98.7%, respectively. All the flavonoids were identified by HR-ESI-MS, 1D and 2D NMR. Compounds and were firstly isolated from the plant of Results from antioxidant assays showed that rutin () and isoquercitrin () had higher antioxidant capacities compared to L-ascorbic acid as the positive control.
作为非药用部位，[植物名称] Bunge的地上部分在采收时总是被丢弃，这导致了资源的巨大浪费和环境压力。由于其黄酮类化合物含量高及其抗氧化活性特性，[植物名称] Bunge的地上部分可被开发成天然抗氧化剂并用于食品中。采用高速逆流色谱（HSCCC）法，使用由正丁基甲基醚/正丁醇/乙腈/水（3:1:1:20，v/v）组成的两相溶剂系统，首次成功地一次性从[植物名称] Bunge的地上部分分离出五种黄酮类化合物，经鉴定分别归类为芦丁（）、异槲皮苷（）、山柰酚-3-O-α-L-鼠李糖基-（1→6）-β-D-葡萄糖苷（）、山柰酚-3-O-β-D-葡萄糖苷（）和芹菜素-7-O-β-D-葡萄糖苷（）。这些植物提取物的纯度分别为97.3%、99.5%、92.8%、98.1%和98.7%。所有黄酮类化合物均通过高分辨电喷雾电离质谱（HR-ESI-MS）、一维和二维核磁共振（1D和2D NMR）进行鉴定。化合物[具体化合物]首次从[植物名称] Bunge中分离得到。抗氧化试验结果表明，与作为阳性对照的L-抗坏血酸相比，芦丁（）和异槲皮苷（）具有更高的抗氧化能力。
