外源感染后小鼠白血病病毒整合的定量研究。
Quantitative studies of integration of murine leukemia virus after exogenous infection.
作者信息
Chattopadhyay S k, Rowe W P, Levine A S
出版信息
Proc Natl Acad Sci U S A. 1976 Nov;73(11):4095-9. doi: 10.1073/pnas.73.11.4095.
Abstract
Using a [3H]DNA probe prepared from AKR murine leukemia virus, we determined the number of copies of the AKR virus genome integrated into the cellular DNA after exogenous infection of NIH mouse, AKR mouse, and rat cells in tissue culture. NIH mouse cells, which lack a portion of the viral genome (referred to as Gross-AKR specific sequences), incorporated three to four copies of these sequences per haploid genome. AKR cells, in which the Gross-AKR specific sequences are already present as three to four copies per haploid genome, did not shwo any distinct change in copy number after infection. Rat cells, which lack DNA sequences homologous to murine leukemia virus, incorporated one copy of the viral genome per haploid genome. It is inferred that the presence of viral sequences may affect the efficiency of integration of exogenous provirus, and that there may be a limit to the number of copies that can be inserted.
摘要
使用从AKR鼠白血病病毒制备的[3H]DNA探针，我们测定了在组织培养中对NIH小鼠、AKR小鼠和大鼠细胞进行外源感染后，整合到细胞DNA中的AKR病毒基因组的拷贝数。缺乏部分病毒基因组（称为Gross-AKR特异性序列）的NIH小鼠细胞，每个单倍体基因组整合了三到四个这些序列的拷贝。每个单倍体基因组中已经存在三到四个Gross-AKR特异性序列的AKR细胞，在感染后拷贝数没有显示出任何明显变化。缺乏与鼠白血病病毒同源的DNA序列的大鼠细胞，每个单倍体基因组整合了一个病毒基因组拷贝。据推测，病毒序列的存在可能会影响外源前病毒的整合效率，并且可插入的拷贝数可能存在限制。
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Repeated sequences in DNA. Hundreds of thousands of copies of DNA sequences have been incorporated into the genomes of higher organisms.DNA中的重复序列。数以十万计的DNA序列拷贝已被纳入高等生物的基因组中。
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