Manuele Maria Gabriela, Ferraro Graciela, Anesini Claudia
Instituto de la Química y Metabolismo del Fármaco, CONICET, Buenos Aires, Argentina.
Phytother Res. 2008 Nov;22(11):1520-6. doi: 10.1002/ptr.2524.
Tilia species are widely used in Europe as medicinal plants. The selective antiproliferative activity of a Tilia cordata flower dichloromethane extract (DME) on a lymphoma cell line has been reported previously and in order to extend this to other unstudied Tilia species, the effect of Tilia x viridis DME on the proliferation of tumor and normal murine lymphocytes was investigated. The bioguided fractionation of DME yielded a fraction rich in limonene (L), alpha-pinene and beta-pinene, which presented a selective antiproliferative action on tumor lymphocytes (EC(50) on tumor cells: 3.8 +/- 0.2 microg/mL; EC(50) on normal cells: 205 +/- 1.8 microg/mL). While all monoterpenes exhibited this activity, limonene proved to be the most active (EC(50) on tumor cells: 35 +/- 2.0 microg/mL; EC(50) on normal cells: 72 +/- 5.0 microg/mL) also exerting a stimulatory effect on non-mitogen stimulated lymphocytes proliferation (% of stimulation respect to control) (mean +/- SEM): L 10 microg/mL: 25 +/- 1.0%; 20 microg/mL: 38.5 +/- 2.5%; L 40 microg/mL: 41 +/- 0.9%; L 60 microg/mL: 58.5 +/- 3%. T. x viridis may thus constitute a potential source of monoterpenes with immunomodulatory activity.
椴树属植物在欧洲被广泛用作药用植物。此前已有报道称，心叶椴花二氯甲烷提取物（DME）对淋巴瘤细胞系具有选择性抗增殖活性。为了将此研究扩展到其他未研究过的椴树属植物，研究了绿椴DME对肿瘤和正常小鼠淋巴细胞增殖的影响。DME的生物导向分级分离得到了一个富含柠檬烯（L）、α-蒎烯和β-蒎烯的级分，该级分对肿瘤淋巴细胞具有选择性抗增殖作用（对肿瘤细胞的半数有效浓度：3.8±0.2微克/毫升；对正常细胞的半数有效浓度：205±1.8微克/毫升）。虽然所有单萜类化合物都表现出这种活性，但柠檬烯被证明是最具活性的（对肿瘤细胞的半数有效浓度：35±2.0微克/毫升；对正常细胞的半数有效浓度：72±5.0微克/毫升），它还对非丝裂原刺激的淋巴细胞增殖具有刺激作用（相对于对照的刺激百分比）（平均值±标准误）：L 10微克/毫升：25±1.0%；20微克/毫升：38.5±2.5%；L 40微克/毫升：41±0.9%；L 60微克/毫升：58.5±3%。因此，绿椴可能构成具有免疫调节活性的单萜类化合物的潜在来源。
