Scheffler A, Rauwald H W, Kampa B, Mann U, Mohr F W, Dhein S
Klinik für Herzchirurgie, Herzzentrum Leipzig, Strümpellstr. 39, 04289 Leipzig, Germany.
J Ethnopharmacol. 2008 Nov 20;120(2):233-40. doi: 10.1016/j.jep.2008.08.018. Epub 2008 Aug 23.
In Southern Europe Olea europaea leafs are known as a folk remedy for hypertension. Cardiovascular diseases are still the leading causes of morbidity and mortality in industrialized countries with hypertension being one of the main risk factors.
We investigated effects of a commercial Olea europaea leaf extract (OLE) on isolated hearts and cultured cardiomyocytes.
Isolated rabbit hearts were perfused according to the Langendorff technique and connected to a 256-channel epicardial mapping system. Voltage clamp experiments were performed in cultured neonatal rat cardiomyocytes using a perforated-patch technique.
OLE caused a concentration-depended decrease in systolic left ventricular pressure and heart rate as well as an increase in relative coronary flow and a slight, but not significant prolongation of PQ-time. There were no significant changes between the groups in the activation-recovery interval and its dispersion, total activation time, peak-to-peak amplitude, percentage of identical breakthrough-points and similar vectors of local activation. Voltage clamp experiments in cultured neonatal rat cardiomyocytes showed a significant decrease in maximum I(Ca,L) by OLE which was reversible upon wash-out.
OLE suppresses the L-type calcium channel directly and reversibly. Our findings might help to understand the traditional use of OLE in the treatment of cardiovascular disease.
在南欧，油橄榄叶被视为治疗高血压的民间药物。在工业化国家，心血管疾病仍然是发病和死亡的主要原因，高血压是主要危险因素之一。
我们研究了一种市售油橄榄叶提取物（OLE）对离体心脏和培养心肌细胞的作用。
采用Langendorff技术灌注离体兔心脏，并连接到256通道心外膜标测系统。使用穿孔膜片钳技术在培养的新生大鼠心肌细胞中进行电压钳实验。
OLE导致左心室收缩压和心率呈浓度依赖性降低，相对冠状动脉血流量增加，PQ间期轻度延长但不显著。各组之间在激活-恢复间期及其离散度、总激活时间、峰峰值幅度、相同突破点百分比和局部激活相似向量方面无显著变化。培养的新生大鼠心肌细胞的电压钳实验显示，OLE使最大L型钙电流（I(Ca,L)）显著降低，洗脱后可逆转。
OLE直接且可逆地抑制L型钙通道。我们的研究结果可能有助于理解OLE在治疗心血管疾病中的传统用途。
