Singh-Naz N, Naz R K
J Clin Microbiol. 1986 May;23(5):840-2. doi: 10.1128/jcm.23.5.840-842.1986.
Monoclonal antibodies were prepared against enteric adenovirus by fusing P3-NS1/-Ag4-1 mouse myeloma cells with lymphocytes from BALB/c mice immunized with enteric adenovirus 40 (Ad40) G2297. Of the several putative clones secreting antibodies to adenovirus, five were found to react specifically to the enteric adenovirus. The specificity of two of these monoclones which recognize a single antigen of a molecular size of 17 kilodaltons was evaluated against 78 clinical isolates. One monoclone (5D8/2C2) reacted with both Ad40 and Ad41, and the other monoclone (2H6/C11) recognized Ad40 only in an enzyme-linked immunosorbent assay (ELISA). These ELISA results correlated well with those of the specific neutralization test or DNA restriction endonuclease analysis or both. The use of this rapid ELISA with these monoclones will find applications in the diagnosis of enteric adenovirus and should facilitate the epidemiologic studies of enteric adenovirus gastroenteritis.
通过将P3-NS1/-Ag4-1小鼠骨髓瘤细胞与用肠道腺病毒40（Ad40）G2297免疫的BALB/c小鼠的淋巴细胞融合，制备了针对肠道腺病毒的单克隆抗体。在几个分泌腺病毒抗体的假定克隆中，发现有五个与肠道腺病毒发生特异性反应。针对78株临床分离株评估了这两种识别分子大小为17千道尔顿单一抗原的单克隆抗体的特异性。一种单克隆抗体（5D8/2C2）与Ad40和Ad41都发生反应，而另一种单克隆抗体（2H6/C11）在酶联免疫吸附测定（ELISA）中仅识别Ad40。这些ELISA结果与特异性中和试验或DNA限制性内切酶分析或两者的结果高度相关。使用这些单克隆抗体进行这种快速ELISA将在肠道腺病毒的诊断中得到应用，并应有助于肠道腺病毒胃肠炎的流行病学研究。
