Snopková S, Götz F, Doskar J, Rosypal S
Department of Genetics and Molecular Biology, Faculty of Science, Masaryk University, Brno, Czech Republic.
FEMS Microbiol Lett. 1994 Dec 1;124(2):131-9. doi: 10.1016/0378-1097(94)90240-2.
The genomes of 47 coagulase-negative staphylococcal strains assigned to different species were analysed by pulsed-field electrophoresis. The strains were clustered on the basis of their similarity in the SmaI restriction patterns into various groups, each group consisting of the type strain and the strains whose SmaI restriction patterns were similar to that of the type of strain. The SmaI restriction groups seem to correspond to the following species: Staphylococcus warneri, S. hominis, S. xylosus, S. lugdunensis, S. kloosii, S. haemolyticus, S. lentus, S. cohnii, S. equorum, S. chromogenes, S. saprophyticus, S. simulans, S. carnosus, S. capitis and S. auricularis. The species S. sciuri, S. caseolyticus, S. gallinarum, S. epidermidis and S. schleiferi were represented only by their type strains and showed no similarity in their SmaI restriction patterns neither to each other nor to all the other species investigated here. Thus, the classification of coagulase-negative staphylococcal strains into the above species seems to be confirmed also by genome restriction analysis carried out by pulsed-field gel electrophoresis.
采用脉冲场凝胶电泳技术对47株分属于不同种的凝固酶阴性葡萄球菌菌株的基因组进行了分析。根据菌株在SmaI酶切图谱上的相似性将其聚类为不同的组，每组包括模式菌株以及SmaI酶切图谱与模式菌株相似的菌株。SmaI酶切组似乎与以下菌种相对应：沃氏葡萄球菌、人葡萄球菌、木糖葡萄球菌、路邓葡萄球菌、克氏葡萄球菌、溶血葡萄球菌、缓慢葡萄球菌、科氏葡萄球菌、马胃葡萄球菌、产色葡萄球菌、腐生葡萄球菌、模仿葡萄球菌、肉葡萄球菌、头葡萄球菌和耳葡萄球菌。松鼠葡萄球菌、解脲葡萄球菌、鸡葡萄球菌、表皮葡萄球菌和施氏葡萄球菌仅由其模式菌株代表，它们的SmaI酶切图谱彼此之间以及与这里研究的所有其他菌种均无相似性。因此，通过脉冲场凝胶电泳进行的基因组限制分析似乎也证实了将凝固酶阴性葡萄球菌菌株分类为上述菌种的分类方法。
