用于葡萄球菌鉴定的MicroScan快速阳性组合鉴定板评估
Evaluation of MicroScan Rapid Pos Combo panels for identification of staphylococci.
作者信息
Stoakes L, Schieven B C, Ofori E, Ewan P, Lannigan R, Hussain Z
机构信息
Department of Clinical Microbiology, Victoria Hospital, London, Ontario, Canada.
出版信息
J Clin Microbiol. 1992 Jan;30(1):93-5. doi: 10.1128/jcm.30.1.93-95.1992.
Abstract
MicroScan Rapid Pos Combo panels (Baxter Diagnostics, Inc., MicroScan, West Sacramento, Calif.) contain substrates conjugated with fluorophores and substrates with a fluorescent pH indicator. AutoSCAn W/A, an automated panel processor equipped with a fluorometer, reads the panels after 2 h of incubation and can identify staphylococci to the species level. We tested 239 strains belonging to 17 species of staphylococci. All the strains were identified by conventional methods (W.E. Kloos and K.H. Schleifer, J. Clin. Microbiol. 1:82-88, 1975) and by the MicroScan Rapid ID system. The system correctly identified 219 (91.6%) strains; nine (3.8%) identification results were probably correct, and six (2.5%) results were incorrect. The system designated five (2.1%) strains as rare biotypes. The automated MicroScan Rapid ID system is useful and reliable in identifying most human isolates of staphylococci encountered in the clinical laboratory.
摘要
MicroScan快速阳性组合板（百特诊断公司，MicroScan，加利福尼亚州西萨克拉门托）包含与荧光团结合的底物以及带有荧光pH指示剂的底物。配备荧光计的自动平板处理器AutoSCAn W/A在孵育2小时后读取平板，并可将葡萄球菌鉴定到种水平。我们测试了属于17种葡萄球菌的239株菌株。所有菌株均通过传统方法（W.E.克洛斯和K.H.施莱费尔，《临床微生物学杂志》1:82 - 88，1975年）和MicroScan快速鉴定系统进行鉴定。该系统正确鉴定了219株（91.6%）菌株；9株（3.8%）的鉴定结果可能正确，6株（2.5%）的结果错误。该系统将5株（2.1%）菌株指定为罕见生物型。自动MicroScan快速鉴定系统在鉴定临床实验室中遇到的大多数人类葡萄球菌分离株方面是有用且可靠的。
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