Westblom T U, Madan E, Midkiff B R
Department of Medicine, St. Louis University School of Medicine, Missouri 63104.
J Clin Microbiol. 1991 Apr;29(4):819-21. doi: 10.1128/jcm.29.4.819-821.1991.
We developed a new agar, egg yolk emulsion (EYE) agar, for cultivation of Helicobacter pylori. EYE agar contains Columbia agar base (Oxoid), 10% EYE (Oxoid), 1% IsoVitaleX (BBL), and 40 mg of Triphenyleteraxolium chloride (Sigma) per liter. We compared EYE agar with the following agars: (i) brain heart infusion agar-7% horse blood-1% IsoVitaleX (GDW agar; C. S. Goodwin, E. D. Blincow, J. R. Warren, T. E. Waters, C. R. Sanderson, and L. Easton, J. Clin. Pathol. 38:1127-1131, 1985), (ii) brain heart infusion agar-10% horse serum-0.2% charcoal-1% yeast extract-40 mg of triphenyltetrazolium chloride per liter (GLU agar; Y. Glupczynski, M. Labbe, and F. Thiabaumont, p. 3-6, in F. Megraud and H. Lamouliatte, ed., Gastroduodenal Pathology and Campylobacter pylori, 1989), (iii) Columbia agar with 7% lysed horse blood (D&M agar; J. C. Dent and C. A. M. McNulty, Eur. J. Clin. Microbiol. Infect. Dis. 7:555-558, 1988), and (iv) brain heart infusion agar-10% EYE-1% IsoVitaleX (BHIE agar). H. pylori CFU counts, expressed as average percentages of maximum growth, were as follows: EYE agar, 96; GDW agar. 76; BHIE agar, 57; D&M agar, 52; and GLU agar, 23. Colony counts for EYE agar were significantly higher than for GDW agar (P = 0.027), BHIE agar (P = 0.005), D&M agar (P = 0.0001), and GLU agar (P less than 0.0001). EYE agar also had higher CFU counts than two commercial chocolate media; the EYE agar count was 80%, versus 33% for BBL chocolate medium and 63% for Remel chocolate medium.
我们研发了一种用于培养幽门螺杆菌的新型琼脂——蛋黄乳液（EYE）琼脂。EYE琼脂含有哥伦比亚琼脂基础培养基（Oxoid公司）、10%的EYE（Oxoid公司）、1%的IsoVitaleX（BBL公司）以及每升40毫克的氯化三苯基四氮唑（Sigma公司）。我们将EYE琼脂与以下几种琼脂进行了比较：（i）脑心浸液琼脂 - 7%马血 - 1% IsoVitaleX（GDW琼脂；C.S.古德温、E.D.布林考、J.R.沃伦、T.E.沃特斯、C.R.桑德森和L.伊斯顿，《临床病理学杂志》38:1127 - 1131，1985年），（ii）脑心浸液琼脂 - 10%马血清 - 0.2%木炭 - 1%酵母提取物 - 每升40毫克氯化三苯基四氮唑（GLU琼脂；Y.格鲁普钦斯基、M.拉贝和F.蒂亚博蒙特，第3 - 6页，载于F.梅格劳德和H.拉穆利亚特编，《胃十二指肠病理学与幽门弯曲菌》，1989年），（iii）含7%裂解马血的哥伦比亚琼脂（D&M琼脂；J.C.登特和C.A.M.麦克纳尔蒂，《欧洲临床微生物学与感染疾病杂志》7:555 - 558，1988年），以及（iv）脑心浸液琼脂 - 10% EYE - 1% IsoVitaleX（BHIE琼脂）。幽门螺杆菌的菌落形成单位（CFU）计数，以最大生长量的平均百分比表示，结果如下：EYE琼脂为96%；GDW琼脂为76%；BHIE琼脂为57%；D&M琼脂为52%；GLU琼脂为23%。EYE琼脂的菌落计数显著高于GDW琼脂（P = 0.027）、BHIE琼脂（P = 0.005）、D&M琼脂（P = 0.0001）和GLU琼脂（P < 0.0001）。EYE琼脂的CFU计数也高于两种商用巧克力培养基；EYE琼脂的计数为80%，而BBL巧克力培养基为33%，Remel巧克力培养基为63%。
