Simon D M, Koenig G, Trenholme G M
Department of Medicine, Rush-Presbyterian-St. Luke's Medical Center, Chicago, IL 60612.
J Infect Dis. 1991 Oct;164(4):800-2. doi: 10.1093/infdis/164.4.800.
Bacterial products, such as endotoxin, activate mononuclear cells to produce tumor necrosis factor (TNF) and other monokines capable of producing host cell injury. THP-1 cell TNF release in response to bacterial products generated during antibiotic killing of Escherichia coli (ATCC 12014) was evaluated. THP-1 is a mature monocytic leukemia cell line that produces TNF in a dose-dependent fashion in response to purified endotoxin. E. coli were incubated in the presence of amikacin, ciprofloxacin, ceftazidime, cefotaxime, aztreonam, or imipenem at concentrations that killed greater than 99.9% of the organisms. Aliquots of these antibiotic-bacterial cultures were added to THP-1 cells, and TNF concentrations were determined by specific immunoassay. Amikacin and imipenem produced rapid bacterial killing and were associated with low TNF levels. Ceftazidime, aztreonam, and cefotaxime killed E. coli at a slower rate and were associated with significant increases in mononuclear cell TNF responses. Ciprofloxacin produced intermediate TNF levels. Differences exist among bactericidal antibodies in their ability to generate products capable of stimulating mononuclear cell TNF release.
细菌产物，如内毒素，可激活单核细胞产生肿瘤坏死因子（TNF）和其他能够造成宿主细胞损伤的单核因子。评估了THP-1细胞对大肠杆菌（ATCC 12014）抗生素杀菌过程中产生的细菌产物的TNF释放情况。THP-1是一种成熟的单核细胞白血病细胞系，对纯化的内毒素以剂量依赖方式产生TNF。将大肠杆菌在阿米卡星、环丙沙星、头孢他啶、头孢噻肟、氨曲南或亚胺培南存在的情况下进行培养，这些抗生素的浓度能杀灭超过99.9%的细菌。将这些抗生素 - 细菌培养物的等分试样加入到THP-1细胞中，并通过特异性免疫测定法测定TNF浓度。阿米卡星和亚胺培南能迅速杀灭细菌，并与低TNF水平相关。头孢他啶、氨曲南和头孢噻肟杀灭大肠杆菌的速度较慢，并与单核细胞TNF反应的显著增加相关。环丙沙星产生的TNF水平处于中等。杀菌性抗体在产生能够刺激单核细胞TNF释放的产物的能力方面存在差异。
