Department of Enviromental Chemistry, IQAB-CSIC C/Jordi Girona 18-26, 08034 Barcelona, Spain.
Talanta. 2004 Feb 27;62(3):549-58. doi: 10.1016/j.talanta.2003.08.022.
Several toxicity procedures are currently being used for the wastewater toxicity assessment. We have undertaken an interlaboratory comparison of the use of different bioluminescence inhibition toxicity tests based on Vibrio fischeri, in order to evaluate their reproducibility for the rapid wastewater toxicity assessment. Twenty-two laboratories took part in this study organized by the Institut Català de Tecnologia (ICT) and the Consejo Superior de Investigaciones Cientificas (CSIC). During the exercise, six series of six samples were analyzed along 5 months. Every batch of samples was composed by three real samples and three standard solutions. The real samples were: an untreated effluent of a paper industry, a sample from a first settlement of a wastewater treatment plant (WWTP) and the final effluent of the WWTP. The goals of the interlaboratory study were to evaluate the repeatability (r) and reproducibility (R) when different laboratories conduct the test, the influence of different matrix samples, the variability between different tests based on the same principle: the bioluminescence inhibition of V. fischeri, but involving different commercial devices and to determine the rate at which participating laboratories successfully completed tests initiated. The maximum number of outlier values was corresponding to a non-treated effluent from a paper industry. This also was the most complex and toxic sample analyzed. An increase on the non-convergent values obtained for the participants was observed at higher matrix complexity and at lower toxicity level. In comparison with other editions of this interlaboratory study the matrixes of real samples analyzed were more complex, nevertheless the final variability coefficient for the exercise was nearby to the average value for the past editions. Due to the high complexity of some samples involved in this intercalibration the stability of real samples were also followed during the test. On the other hand, no relation was found between final results and the different devices, as show the cluster analysis.
目前，有几种毒性程序可用于废水毒性评估。我们已经对基于发光菌（Vibrio fischeri）的不同生物发光抑制毒性测试的使用进行了实验室间比较，以评估它们在快速废水毒性评估中的重现性。共有 22 家实验室参加了由加泰罗尼亚技术研究所（Institut Català de Tecnologia，ICT）和西班牙国家研究委员会（Consejo Superior de Investigaciones Científicas，CSIC）组织的这项研究。在实验过程中，6 组 6 个样本在 5 个月内进行了分析。每批样本由三个实际样本和三个标准溶液组成。实际样本为：未经处理的造纸厂废水、废水处理厂一级沉降物和废水处理厂最终出水。实验室间研究的目的是评估不同实验室进行测试时的重复性（r）和再现性（R）、不同基质样本的影响、基于相同原理（发光菌的生物发光抑制）但涉及不同商业设备的不同测试之间的可变性，并确定参与实验室成功完成启动测试的速度。最大数量的异常值对应于未经处理的造纸厂废水。这也是分析的最复杂和毒性最大的样本。随着基质复杂性的增加和毒性水平的降低，参与者获得的非一致性值增加。与该实验室间研究的其他版本相比，分析的实际样本基质更加复杂，但整个实验的最终变异系数接近过去版本的平均值。由于参与此次内部校准的一些样本的复杂性很高，因此在测试过程中还对实际样本的稳定性进行了跟踪。另一方面，聚类分析表明，最终结果与不同的设备之间没有关系。
