Key Laboratory of Magnetic Molecules & Magnetic Information Materials Ministry of Education, Shanxi Normal University, Linfen 041004, Shanxi, China; School of Chemical and Material Science, Shanxi Normal University, Linfen 041004, Shanxi, China.
School of Chemical and Material Science, Shanxi Normal University, Linfen 041004, Shanxi, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2017 Aug 5;183:103-108. doi: 10.1016/j.saa.2017.04.009. Epub 2017 Apr 20.
The famous weak chemiluminescence (CL) system of potassium permanganate and sodium bisulfite (KMnO-HSO) was enhanced by the hollow fluorescent carbon nanodots (HCNs). The investigation of mechanism revealed that the enhanced CL was induced by the excited-state HCNs (HCNs), which could be produced from the electron-transfer annihilation of positively charged HCNs (HCNs) and negatively charged HCNs (HCNs) as well as by CL resonance energy transfer (CRET) from excited SO (SO)/O to HCNs. The dihydralazine sulfate (DHZS) had a diminishing effect on the CL of HCNs-KMnO-HSO system due to the competitive consumption of O. Under the optimal conditions, the reduced CL signal with the concentration of DHZS was linear in the range of 1.0×10-7.0×10mol/L with a detection limit of 3.0×10mol/L. The relative standard deviation for seven repeated determination of 5.0×10mol/L DHZS was 2.1%. The established method was applied to the determination of DHZS in pharmaceutical preparations, human urine and plasma samples with good precision and accuracy.
高锰酸钾-亚硫酸氢钠（KMnO-HSO）的弱化学发光（CL）体系被中空荧光碳纳米点（HCNs）增强。机理研究表明，增强的 CL 是由激发态 HCNs（HCNs）诱导的，激发态 HCNs（HCNs）可以通过带正电荷的 HCNs（HCNs）和带负电荷的 HCNs（HCNs）的电子转移湮灭以及从激发态 SO（SO）/O 到 HCNs 的 CL 共振能量转移（CRET）产生。二氢氯嗪硫酸盐（DHZS）由于 O 的竞争消耗，对 HCNs-KMnO-HSO 体系的 CL 有抑制作用。在最佳条件下，随着 DHZS 浓度的降低，CL 信号呈线性关系，在 1.0×10-7.0×10mol/L 范围内，检出限为 3.0×10mol/L。7 次重复测定 5.0×10mol/L DHZS 的相对标准偏差为 2.1%。该方法应用于药物制剂、人尿和血浆样品中 DHZS 的测定，具有良好的精密度和准确度。
