Kim Jungmin, Kang Hee Young, Lee Yeonhee
Department of Microbiology, School of Medicine, Kyungpook National University, Daegu 702-701, Republic of Korea.
J Microbiol. 2008 Oct;46(5):478-81. doi: 10.1007/s12275-008-0150-y. Epub 2008 Oct 31.
From water samples collected monthly between 2000 and 2001 from the Han River in Seoul, sixteen strains of Escherichia coli which confer resistance to at least 10 kinds of antimicrobial agents were isolated. From these isolates, 2 kinds of extended-spectrum beta-lactamases (ESBLs) and one plasmid-mediated AmpC beta-lactamase were detected; CTX-M-14 from 10 isolates, TEM-52 from 5 isolates, and CMY-1 from one isolate. Class 1 integron gene cassettes, such as aadA1, dfr12-orfF-aadA2, and dfr17-aadA5, were also detected and the integrons are the same as those found in E. coli isolated from swine, poultry, and humans in Korea. The result of this study indicated the importance of river water as a reservoir for antimicrobial resistance genes and resistant bacteria.
2000年至2001年期间，每月从首尔汉江采集水样，从中分离出16株对至少10种抗菌药物具有抗性的大肠杆菌菌株。从这些分离株中，检测到2种超广谱β-内酰胺酶（ESBLs）和1种质粒介导的AmpCβ-内酰胺酶；10株分离株中检测到CTX-M-14，5株分离株中检测到TEM-52，1株分离株中检测到CMY-1。还检测到1类整合子基因盒，如aadA1、dfr12-orfF-aadA2和dfr17-aadA5，这些整合子与在韩国从猪、家禽和人类分离出的大肠杆菌中发现的整合子相同。本研究结果表明河水作为抗菌抗性基因和抗性细菌储存库的重要性。
