Rosenblatt J E, Gerdts A M
Antimicrob Agents Chemother. 1977 Jul;12(1):37-9. doi: 10.1128/AAC.12.1.37.
The in vitro inhibitory activity of spectinomycin was tested against various anaerobic bacteria. Different results were obtained with different media and with different initial pH's of the media. The highest minimum inhibitory concentrations for Bacteroides fragilis ([Formula: see text] 128 mug/ml) were obtained with the use of Wilkins-Chalgren agar (pH 7.2) and Brucella blood agar (pH 7.0). Brucella blood agar at higher pH's (7.4 and 8.0) and Mueller-Hinton and Diagnostic Sensitivity Test agars produced lower minimum inhibitory concentrations (32 and 64 mug/ml). This same relationship between spectinomycin activity and pH of the medium was, in general, observed with these media and other anaerobes, including isolates of B. melaninogenicus, Fusobacterium, gram-positive cocci, Clostridium perfringens, and C. ramosum. The variable results observed in this study and in two others make it difficult to predict the clinical usefulness of spectinomycin in the treatment of anaerobic infections. It is probably most appropriate to be guided by results obtained with Wilkins-Chalgren agar and the method proposed as a reference to the National Committee for Clinical Laboratory Standards. These results indicate that spectinomycin is not a potent inhibitor of B. fragilis or other clinically significant anaerobes.
对壮观霉素针对各种厌氧菌的体外抑制活性进行了测试。使用不同的培养基以及不同初始pH值的培养基得到了不同的结果。使用威尔金斯-查尔格伦琼脂（pH 7.2）和布鲁氏菌血琼脂（pH 7.0）时，脆弱拟杆菌的最低抑菌浓度最高（[公式：见正文]128μg/ml）。较高pH值（7.4和8.0）的布鲁氏菌血琼脂以及穆勒-欣顿琼脂和诊断敏感性试验琼脂产生的最低抑菌浓度较低（32和64μg/ml）。对于这些培养基以及其他厌氧菌，包括产黑素拟杆菌、梭杆菌、革兰氏阳性球菌、产气荚膜梭菌和多枝梭菌的分离株，一般都观察到壮观霉素活性与培养基pH值之间存在这种相同的关系。在本研究以及其他两项研究中观察到的结果不一，使得难以预测壮观霉素在治疗厌氧菌感染中的临床实用性。以威尔金斯-查尔格伦琼脂获得的结果以及被提议作为美国国家临床实验室标准委员会参考方法的结果为指导可能最为合适。这些结果表明，壮观霉素不是脆弱拟杆菌或其他临床上重要厌氧菌的有效抑制剂。
