Said J W, Sassoon A F, Shintaku I P, Kurtin P J, Pinkus G S
Department of Pathology, Cedars Sinai Medical Center, Los Angeles, CA 90048.
Am J Pathol. 1991 Feb;138(2):261-4.
Recent evidence suggests that nodular lymphocyte predominance Hodgkin's disease (NLPHD) is a distinct entity that may be related to progressively transformed germinal centers, abnormal B-lymphoid hyperplasia, and low-grade B-cell lymphoma. bcl-2 is a marker for the translocation t(14;18)(q32;q21), which occurs in most follicular-derived B-cell lymphomas. Eleven cases of NLPHD and 19 cases of Hodgkin's disease of nodular sclerosis (NSHD) and mixed cellularity (MCHD) type were analyzed for immunoglobulin JH gene rearrangement. bcl-2 translocation was determined with Southern blot analysis and the polymerase chain reaction using biotin labeled probes to the major breakpoint region and the alkaline phosphatase reaction. All cases of NLPHD were negative for JH gene rearrangement and bcl-2 translocation. Cases of NSHD and MCHD were similarly negative for bcl-2, although three cases exhibited clonal JH gene rearrangements. These results confirm that a clonal B-cell population is not detected in NLPHD. Cases of NLPHD differ from most low-grade follicular B-cell lymphomas in that they lack bcl-2 gene rearrangement and t(14;18) translocation at the major breakpoint region.
最近的证据表明，结节性淋巴细胞为主型霍奇金淋巴瘤（NLPHD）是一种独特的实体，可能与进行性转化的生发中心、异常B淋巴细胞增生以及低度B细胞淋巴瘤有关。bcl-2是t(14;18)(q32;q21)易位的标志物，该易位发生在大多数滤泡来源的B细胞淋巴瘤中。对11例NLPHD以及19例结节硬化型霍奇金淋巴瘤（NSHD）和混合细胞型霍奇金淋巴瘤（MCHD）进行免疫球蛋白JH基因重排分析。采用Southern印迹分析以及使用生物素标记探针针对主要断裂点区域的聚合酶链反应和碱性磷酸酶反应来确定bcl-2易位情况。所有NLPHD病例的JH基因重排和bcl-2易位均为阴性。NSHD和MCHD病例的bcl-2同样为阴性，尽管有3例表现出克隆性JH基因重排。这些结果证实，在NLPHD中未检测到克隆性B细胞群体。NLPHD病例与大多数低度滤泡性B细胞淋巴瘤不同，在于它们在主要断裂点区域缺乏bcl-2基因重排和t(14;18)易位。
