Fan Yuwei, Sun Zhi, Moradian-Oldak Janet
Center for Craniofacial Molecular Biology, University of Southern California, 2250 Alcazar Street, CSA 103, HSC, Los Angeles, CA 90033, USA.
Biomaterials. 2009 Feb;30(4):478-83. doi: 10.1016/j.biomaterials.2008.10.019. Epub 2008 Nov 8.
Reconstructing enamel-like structures on teeth have been an important topic of study in the material sciences and dentistry. The important role of amelogenin in modulating the mineralization of organized calcium phosphate crystals has been previously reported. We used amelogenin and utilized a modified biomimetic deposition method to remineralize the surface of etched enamel to form mineral layers containing organized needle-like fluoridated hydroxyapatite crystals. The effect of a recombinant amelogenins (rP172) on the microstructure of the mineral in the coating was analyzed by SEM, XRD and FT-IR. At rP172 concentrations below 33 microg/mL, no significant effect was observed. In the presence of 1 mg/L F and at a concentration of 33 microg/mL rP172, formation of fused crystals growing from the enamel surface was initiated. Amelogenin promoted the oriented bundle formation of needle-like fluoridated hydroxyapatite in a dose dependent manner. Biomimetic synthesis of the amelogenin fluoridated hydroxyapatite nano-composite is one of the primary steps towards the development and design of novel biomaterial for future application in reparative and restorative dentistry.
在牙齿上重建类釉质结构一直是材料科学和牙科学领域的重要研究课题。以往的研究报道了釉原蛋白在调节有序磷酸钙晶体矿化过程中的重要作用。我们使用釉原蛋白，并采用改良的仿生沉积方法对酸蚀釉质表面进行再矿化，以形成含有有序针状氟化羟基磷灰石晶体的矿化层。通过扫描电子显微镜（SEM）、X射线衍射（XRD）和傅里叶变换红外光谱（FT-IR）分析了重组釉原蛋白（rP172）对涂层中矿物质微观结构的影响。当rP172浓度低于33微克/毫升时，未观察到明显影响。在1毫克/升氟存在且rP172浓度为33微克/毫升的情况下，开始形成从釉质表面生长的融合晶体。釉原蛋白以剂量依赖的方式促进针状氟化羟基磷灰石的定向束状形成。釉原蛋白氟化羟基磷灰石纳米复合材料的仿生合成是开发和设计新型生物材料的主要步骤之一，有望在未来的修复和补牙牙科中得到应用。
