Ruan Qichao, Siddiqah Nadia, Li Xiaochen, Nutt Steven, Moradian-Oldak Janet
Center for Craniofacial Molecular Biology, Herman Ostrow School of Dentistry, University of Southern California , Los Angeles, CA , USA and.
Connect Tissue Res. 2014 Aug;55 Suppl 1(0 1):150-4. doi: 10.3109/03008207.2014.923856.
We recently reported an amelogenin-chitosan (CS-AMEL) hydrogel as a promising biomimetic material for future in situ human enamel regrowth. To further optimize the necessary conditions for clinical applicability of CS-AMEL hydrogel, herein we studied the effects of viscosity and supersaturation degree on the size and orientation of synthetic crystals by means of scanning electron microscopy (SEM) and X-ray diffraction (XRD). Raising the hydrogel viscosity by increasing chitosan concentration from 1% to 2% (w/v) improved the orientation of the crystals, while a higher supersaturation (σ(HAp) >10.06, [Ca(2+)] >5 mM) resulted in the formation of random crystals with larger sizes and irregular structures. We conclude that optimal conditions to produce organized enamel-like crystals in a CS-AMEL hydrogel are: 2% (w/v) chitosan, 2.5 mM calcium, and 1.5 mM phosphate (degree of supersaturation = 8.23) and 200 µg/ml of amelogenin.
我们最近报道了一种牙釉蛋白-壳聚糖（CS-AMEL）水凝胶，它是一种有前景的仿生材料，有望实现未来人类牙釉质的原位再生。为了进一步优化CS-AMEL水凝胶临床应用的必要条件，在此我们通过扫描电子显微镜（SEM）和X射线衍射（XRD）研究了粘度和过饱和度对合成晶体尺寸和取向的影响。将壳聚糖浓度从1%（w/v）提高到2%来增加水凝胶粘度，可改善晶体的取向，而更高的过饱和度（σ(HAp) >10.06，[Ca(2+)] >5 mM）会导致形成尺寸更大且结构不规则的随机晶体。我们得出结论，在CS-AMEL水凝胶中产生有序牙釉质样晶体的最佳条件是：2%（w/v）壳聚糖、2.5 mM钙、1.5 mM磷酸盐（过饱和度 = 8.23）以及200 μg/ml牙釉蛋白。
