Department of Applied Biological Science, Tokyo Noko University, Fuchu, Saiwai-cho 3-5-8, Tokyo, 183-8509, Japan.
Cytotechnology. 2000 Jul;33(1-3):229-35. doi: 10.1023/A:1008141918852.
Actions of chlorogenic acid, a major component of coffee, andits constituents, caffeic and quinic acids, on theproliferation and invasion of AH109A, a rat ascites hepatomacell line, were investigated using in vitro assay systems. Allthree components suppressed the AH109A invasion atconcentrations of 5-40 muM without altering the cellproliferation. At the concentration of 10 muM, chlorogenic,caffeic and quinic acids significantly (P < 0.05) suppressedthe invasion by 68%, 36% and 31%, respectively, implying thatthe suppressive effect of chlorogenic acid on the AH109Ainvasion might result from the additive effects of itsconstituents, caffeic and quinic acids. At the concentrationof 10 muM, cinnamic acid and p-coumaric acid (4-hydroxycinnamicacid) exerted no or little influence on the invasion, whereascaffeic acid (3,4-dihydroxycinnamic acid) significantly (P <0.05) suppressed it, suggesting the possible involvement ofthe 3,4-dihydroxy group of caffeic acid in the suppression.Chlorogenic acid was thus demonstrated to be one of thechemical entities in coffee suppressing the hepatoma invasionin vitro, and both of its constituents, caffeic and quinicacids, to be responsible for the anti-invasive activity. Theseresults suggest the existence of nutritionally andpharmacologically important substances in coffee which controltumor cell invasion.
咖啡的主要成分之一——绿原酸及其组成部分咖啡酸和奎尼酸对大鼠腹水肝癌细胞系 AH109A 的增殖和侵袭的作用，通过体外检测系统进行了研究。这三种成分在 5-40 μM 的浓度下均能抑制 AH109A 的侵袭，而不改变细胞增殖。在 10 μM 浓度下，绿原酸、咖啡酸和奎尼酸分别显著（P <0.05）抑制侵袭 68%、36%和 31%，表明绿原酸对 AH109A 侵袭的抑制作用可能是其组成部分咖啡酸和奎尼酸的相加作用所致。在 10 μM 浓度下，肉桂酸和对香豆酸（4-羟基肉桂酸）对侵袭没有影响或影响很小，而咖啡酸（3,4-二羟基肉桂酸）则显著（P <0.05）抑制侵袭，表明咖啡酸的 3,4-二羟基可能参与了抑制作用。因此，绿原酸被证明是咖啡中抑制肝癌体外侵袭的化学实体之一，其组成部分咖啡酸和奎尼酸都对其抗侵袭活性负责。这些结果表明咖啡中存在控制肿瘤细胞侵袭的营养和药理上重要的物质。
