National Institute of Vegetable and Tea Sciences, National Agriculture and Food Research Organization, 2769 Kanaya, Shimada, Shizuoka, 428-8501, Japan,
Cytotechnology. 2007 Dec;55(2-3):135-42. doi: 10.1007/s10616-007-9112-1. Epub 2007 Nov 25.
'Benifuuki', a tea (Camellia Sinensis L.) cultivar in Japan, is rich in anti-allergic epigallocatechin-3-O-(3-O-methyl) gallate (EGCG3''Me). 'Benifuuki' green tea and simultaneous addition of ginger extract remarkably suppressed cytokine (TNF-alpha and MIP-1alpha) secretion from mouse bone marrow-derived mast cells after antigen stimulation and, as expected, suppressed delay-type allergy. After drinking 'benifuuki' green tea containing 43.5 mg of EGCG and 8.5 mg of EGCG3''Me, the AUC (area under the drug concentration time curve; min mug/ml) of EGCG was 6.72 +/- 2.87 and EGCG3''Me was 8.48 +/- 2.54 in healthy human volunteers. Though the dose of EGCG was 5.1 times the dose of EGCG3''Me, the AUC of EGCG3''Me was higher than that of EGCG. A double blind clinical study on subjects with Japanese cedar pollinosis was carried out. At the 11th week after starting the study, in the most severe cedar pollen scattering period, symptoms, i.e., blowing the nose and itching eyes, were significantly relieved in the 'benifuuki' intake group compared with the placebo group, and blowing the nose, itching eyes and nasal symptom score, and at the 11th and 13th weeks, stuffy nose, throat pain and the nasal symptom medication score were significantly relieved in the 'benifuuki' containing ginger extract group compared with the placebo group. These results suggested that over one consecutive month, drinking 'benifuuki' green tea was useful to reduce some of the symptoms from Japanese cedar pollinosis, and did not affect any normal immune response in subjects with seasonal rhinitis, and the ginger extract enhanced the effect of 'benifuuki' green tea.
日本的“弁财天”茶（Camellia Sinensis L.）富含抗过敏表没食子儿茶素-3-O-(3-O-甲基)没食子酸酯（EGCG3''Me）。“弁财天”绿茶和同时添加生姜提取物显著抑制了抗原刺激后小鼠骨髓来源肥大细胞细胞因子（TNF-α 和 MIP-1α）的分泌，并且如预期的那样，抑制了迟发型过敏。在健康志愿者中饮用含有 43.5 毫克 EGCG 和 8.5 毫克 EGCG3''Me 的“弁财天”绿茶后，EGCG 的 AUC（药物浓度时间曲线下面积；min mug/ml）为 6.72 +/- 2.87，EGCG3''Me 为 8.48 +/- 2.54。尽管 EGCG 的剂量是 EGCG3''Me 的 5.1 倍，但 EGCG3''Me 的 AUC 高于 EGCG。在患有日本雪松花粉症的受试者中进行了一项双盲临床试验。在研究开始后的第 11 周，在最严重的雪松花粉散射期间，与安慰剂组相比，“弁财天”摄入组的症状（打喷嚏和眼睛瘙痒）明显缓解，并且在第 11 周和第 13 周，打喷嚏、眼睛瘙痒和鼻部症状评分以及鼻塞、喉咙痛和鼻部症状药物评分均明显缓解。在含有生姜提取物的“弁财天”组与安慰剂组相比。这些结果表明，连续饮用“弁财天”绿茶一个月以上有助于减轻一些日本雪松花粉症的症状，并且不会影响季节性鼻炎患者的任何正常免疫反应，生姜提取物增强了“弁财天”绿茶的效果。
