Gorris L G, Voet A C, van der Drift C
Department of Microbiology, Faculty of Science, University of Nijmegen, The Netherlands.
Biofactors. 1991 Jan;3(1):29-35.
Archaeoglobus fulgidus is an extremely thermophilic, sulphate reducing archaebacterium thought to represent a biochemical missing-link between sulphur-metabolizing bacteria and methanogenic bacteria. Whereas the phylogenetic position of A.fulgidus is closer to the sulphur-metabolizing bacteria, there is a partial overlap in the biochemical machinery of A.fulgidus with both groups of bacteria. In particular, the presence of a number of aberrant cofactors up to now thought to be involved exclusively in the process of methanogenesis in methanogenic archaebacteria, i.e. coenzyme F420, methanofuran and methanopterin, has been indicated by previous studies. Here we present evidence for the structural identity of the methanopterin cofactor of A.fulgidus with the methanopterin isolated from Methanobacterium thermoautotrophicum and show that this non-methanogenic bacterium contains two as yet unknown analogues of coenzyme F420. The levels of the various cofactors were determined in cultures grown either on formate or lactate as the carbon source and sulphate or thiosulphate as the sulphur source.
嗜热栖热袍菌是一种极端嗜热的硫酸盐还原古细菌，被认为代表了硫代谢细菌和产甲烷细菌之间的一个生化缺失环节。尽管嗜热栖热袍菌的系统发育位置更接近硫代谢细菌，但它的生化机制与这两类细菌都有部分重叠。特别是，先前的研究表明，嗜热栖热袍菌中存在一些异常辅因子，这些辅因子迄今被认为仅参与产甲烷古细菌的产甲烷过程，即辅酶F420、甲烷呋喃和甲蝶呤。在此，我们提供证据表明嗜热栖热袍菌的甲蝶呤辅因子与从嗜热自养甲烷杆菌中分离出的甲蝶呤在结构上相同，并表明这种非产甲烷细菌含有两种尚未知的辅酶F420类似物。在以甲酸盐或乳酸盐作为碳源、硫酸盐或硫代硫酸盐作为硫源培养的培养物中测定了各种辅因子的水平。
