芳基磺酰四唑，新型偶联试剂以及脱氧核糖寡核苷酸合成三酯法的进一步改进。
Arylsulfonyltetrazoles, new coupling reagents and further improvements in the triester method for the synthesis of deoxyribooligonucleotides.
作者信息
Stawinski J, Hozumi T, Narang S A, Bahl C P, Wu R
出版信息
Nucleic Acids Res. 1977 Feb;4(2):353-71. doi: 10.1093/nar/4.2.353.
Abstract
The modified triester approach has been further improved and refined to the synthesis of defined sequences of deoxyribo-oligonucleotides. Improvements include arylsulfonyltetrazoles as faster and milder condensing agents, benzenesulfonic acid to avoid depurination during deblocking of trityl protecting groups and improved chromatographic procedures for purification of triester intermediates and purification of the final product containing 3'-5' phosphodiester linkages.
摘要
改良三酯法已进一步改进和完善，用于合成特定序列的脱氧核糖寡核苷酸。改进之处包括使用芳基磺酰四唑作为更快、更温和的缩合剂，使用苯磺酸以避免在三苯甲基保护基团脱保护过程中发生脱嘌呤反应，以及改进用于纯化三酯中间体和含有3'-5'磷酸二酯键的最终产物的色谱方法。
Zotero 插件