Efimov V A, Buryakova A A, Reverdatto S V, Chakhmakhcheva O G
Nucleic Acids Res. 1983 Dec 10;11(23):8369-87. doi: 10.1093/nar/11.23.8369.
A modified phosphotriester method has been employed for the efficient chemical synthesis of long-chain deoxyribooligonucleotides. During the course of this work, a general and rapid procedure was developed for the preparation of 24-62-mers in solution. Preparative reversed phase column chromatography on silanized silica gel was used to purify triester intermediates starting from 10-mers. The rapid synthesis of 32-mer and 42-mer on glass and silica gel supports using suitably protected 2-8-mer blocks as coupling units has been also accomplished. In particular, a convenient procedure for the solid-phase synthesis of oligonucleotide blocks bearing 3'-terminal phosphodiester groups is described.
一种改良的磷酸三酯法已被用于高效化学合成长链脱氧核糖寡核苷酸。在这项工作过程中，开发了一种通用且快速的方法来在溶液中制备24至62聚体。使用硅烷化硅胶上的制备型反相柱色谱法从10聚体开始纯化三酯中间体。还完成了使用适当保护的2至8聚体片段作为偶联单元在玻璃和硅胶载体上快速合成32聚体和42聚体。特别地，描述了一种用于固相合成带有3'-末端磷酸二酯基团的寡核苷酸片段的简便方法。
