Pesenti Peter T, Sikaroodi Masoumeh, Gillevet Patrick M, Sánchez-Porro Cristina, Ventosa Antonio, Litchfield Carol D
Department of Environmental Science and Policy, George Mason University, Manassas, VA 20110, USA.
Int J Syst Evol Microbiol. 2008 Dec;58(Pt 12):2710-5. doi: 10.1099/ijs.0.2008/002410-0.
A motile, rod-shaped, pink-pigmented, extremely halophilic archaeon, strain SF3-213(T), was isolated from a crystallizer pond at the Cargill Solar Salt Plant, Newark, California (USA). Analysis of the almost-complete 16S rRNA gene sequence showed that the isolate was phylogenetically related to species of the genus Halorubrum, with a close relationship to Halorubrum trapanicum NRC 34021(T) (98.6 % similarity), Halorubrum sodomense ATCC 33755(T) (98.3 %) and Halorubrum xinjiangense AS 1.3527(T) (98.2 %). The polar lipids of strain SF3-213(T) were C(20)C(20) derivatives of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and a sulfated diglycosyl-diether. Strain SF3-213(T) grew in 2.5-5.0 M NaCl. The temperature and pH ranges for growth were 25-42 degrees C and 6.8-8.5, respectively. Optimal growth occurred at 3.5-4.5 M NaCl, 37 degrees C and pH 7.3. Mg(2+) was required for growth. The DNA G+C content was 69.4 mol%. DNA-DNA hybridization values lower than 70 % were obtained between strain SF3-213(T) and the closely related species of the genus Halorubrum. Based on the data presented in this study, strain SF3-213(T) represents a novel species for which the name Halorubrum californiense sp. nov. is proposed; the type strain is SF3-213(T) (=CECT 7256(T)=DSM 19288(T)=JCM 14715(T)).
从美国加利福尼亚州纽瓦克市嘉吉太阳能盐厂的结晶池中分离出一株运动性、杆状、粉红色色素、极端嗜盐古菌，菌株SF3-213(T)。对几乎完整的16S rRNA基因序列分析表明，该分离株在系统发育上与嗜盐红菌属的物种相关，与特拉帕尼嗜盐红菌NRC 34021(T)（相似度98.6%）、索多玛嗜盐红菌ATCC 33755(T)（98.3%）和新疆嗜盐红菌AS 1.3527(T)（98.2%）关系密切。菌株SF3-213(T)的极性脂类为磷脂酰甘油、磷脂酰甘油磷酸甲酯、磷脂酰甘油硫酸酯和一种硫酸化二糖基二醚的C(20)C(20)衍生物。菌株SF3-213(T)在2.5 - 5.0 M NaCl中生长。生长的温度范围和pH范围分别为25 - 42℃和6.8 - 8.5。在3.5 - 4.5 M NaCl、37℃和pH 7.3时生长最佳。生长需要Mg(2+)。DNA的G+C含量为69.4 mol%。菌株SF3-213(T)与嗜盐红菌属的近缘物种之间的DNA-DNA杂交值低于70%。根据本研究提供的数据，菌株SF3-213(T)代表一个新物种，为此提出新名称加利福尼亚嗜盐红菌（Halorubrum californiense sp. nov.）；模式菌株为SF3-213(T)（=CECT 7256(T)=DSM 19288(T)=JCM 14715(T)）。
