Habib Shafina Hanim Mohd, Makpol Suzana, Abdul Hamid Noor Aini, Das Srijit, Ngah Wan Zurinah Wan, Yusof Yasmin Anum Mohd
Department of Biochemistry, Faculty of Medicine, Medical Centre, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia.
Clinics (Sao Paulo). 2008 Dec;63(6):807-13. doi: 10.1590/s1807-59322008000600017.
To evaluate the effect of ginger extract on the expression of NFkappaB and TNF-alpha in liver cancer-induced rats.
Male Wistar rats were randomly divided into 5 groups based on diet: i) control (given normal rat chow), ii) olive oil, iii) ginger extract (100mg/kg body weight), iv) choline-deficient diet + 0.1% ethionine to induce liver cancer and v) choline-deficient diet + ginger extract (100mg/kg body weight). Tissue samples obtained at eight weeks were fixed with formalin and embedded in paraffin wax, followed by immunohistochemistry staining for NFkappaB and TNF-alpha.
The expression of NFkappaB was detected in the choline-deficient diet group, with 88.3 +/- 1.83% of samples showing positive staining, while in the choline-deficient diet supplemented with ginger group, the expression of NFkappaB was significantly reduced, to 32.35 +/- 1.34% (p<0.05). In the choline-deficient diet group, 83.3 +/- 4.52% of samples showed positive staining of TNF-alpha, which was significantly reduced to 7.94 +/- 1.32% (p<0.05) when treated with ginger. There was a significant correlation demonstrated between NFkappaB and TNF-alpha in the choline-deficient diet group but not in the choline-deficient diet treated with ginger extract group.
In conclusion, ginger extract significantly reduced the elevated expression of NFkappaB and TNF-alpha in rats with liver cancer. Ginger may act as an anti-cancer and anti-inflammatory agent by inactivating NFkappaB through the suppression of the pro-inflammatory TNF-alpha.
评估生姜提取物对肝癌诱导大鼠中核因子κB（NFκB）和肿瘤坏死因子-α（TNF-α）表达的影响。
雄性Wistar大鼠根据饮食随机分为5组：i）对照组（给予正常大鼠饲料），ii）橄榄油组，iii）生姜提取物组（100mg/kg体重），iv）胆碱缺乏饮食+0.1%乙硫氨酸诱导肝癌组，v）胆碱缺乏饮食+生姜提取物组（100mg/kg体重）。八周时获取的组织样本用福尔马林固定并石蜡包埋，随后进行NFκB和TNF-α的免疫组织化学染色。
在胆碱缺乏饮食组中检测到NFκB的表达，88.3±1.83%的样本呈阳性染色，而在补充生姜的胆碱缺乏饮食组中，NFκB的表达显著降低，降至32.35±1.34%（p<0.05）。在胆碱缺乏饮食组中，83.3±4.52%的样本TNF-α呈阳性染色，用生姜处理后显著降至7.94±1.32%（p<0.05）。胆碱缺乏饮食组中NFκB和TNF-α之间存在显著相关性，但在生姜提取物处理的胆碱缺乏饮食组中不存在。
总之，生姜提取物显著降低了肝癌大鼠中NFκB和TNF-α的升高表达。生姜可能通过抑制促炎因子TNF-α使NFκB失活，从而起到抗癌和抗炎作用。
