Stickler D, Hewett P
School of Pure and Applied Biology, University of Wales, College of Cardiff, UK.
Eur J Clin Microbiol Infect Dis. 1991 May;10(5):416-21. doi: 10.1007/BF01968021.
As part of a programme to establish the relative merits of antiseptics that are used as bladder instillations to control urinary tract infections in patients with indwelling catheters, the activity of five such formulations were tested against dense (10(9) cfu/cm2) mixed biofilms composed of Citrobacter diversus, Pseudomonas aeruginosa and Enterococcus faecalis growing on silicone discs. All three species were resistant to chlorhexidine (200 mg/l) and povidone-iodine (1% v/v) in the biofilm mode of growth, whereas these agents rapidly eliminated viable cells from urine suspensions of the mixed community. Lactic acid (1% v/v) produced a 1 log reduction of the biofilm population within 30 min of exposure. The mandelic acid (1% w/v) and mandelic acid (0.5% w/v)/lactic acid (0.5% v/v) mixture proved to be the most effective in eliminating the biofilm organisms. It is suggested that these latter solutions should now be tested for efficacy in bladder washouts against urinary tract infections in catheterized patients.
作为一项确定用于膀胱灌注以控制留置导尿管患者尿路感染的防腐剂相对优点的计划的一部分，测试了五种此类制剂对在硅胶圆盘上生长的由奇异柠檬酸杆菌、铜绿假单胞菌和粪肠球菌组成的密集（10⁹ cfu/cm²）混合生物膜的活性。在生物膜生长模式下，所有这三种菌种对洗必泰（200 mg/l）和聚维酮碘（1% v/v）均具有抗性，而这些药剂能迅速从混合菌群的尿液悬液中清除活细胞。乳酸（1% v/v）在暴露30分钟内使生物膜菌量减少1个对数级。扁桃酸（1% w/v）和扁桃酸（0.5% w/v）/乳酸（0.5% v/v）混合物被证明在清除生物膜菌方面最为有效。建议现在对后一种溶液在膀胱冲洗中治疗导尿患者尿路感染的疗效进行测试。
