Soares Janir Alves, Leonardo Mário Roberto, da Silva Léa Assed Bezerra, Tanomaru Filho Mário, Ito Izabel Yoko
Discipline of Endodontics, Department of Dentistry, Federal University of Vales do Jequitinhonha e Mucuri, UFVJM, Diamantina, MG, Brazil.
J Appl Oral Sci. 2006 Jun;14(3):172-7. doi: 10.1590/s1678-77572006000300005.
The aim of this study was to evaluate the antiseptic efficacy of rotary instrumentation associated with calcium hydroxide-based pastes prepared with different vehicles and antiseptics. Chronic periapical lesions were experimentally induced in 72 premolar root canals of four dogs. Under controlled asepsis, after initial microbiological sampling (A1), the root canals were instrumented using the ProFile system in conjunction with 5.25% sodium hypochlorite and the intracanal medication was placed. Four experimental groups were formed according to the pastes used: group 1- Calen (n=18), group 2- Calen+CPMC (n=20), group 3- Ca(OH)(2) p.a.+ anaesthetic solution (n=16) and group 4- Ca(OH)(2) p.a.+ 2% chlorhexidine digluconate (n=18). After 21 days, the pastes were removed; the canals were emptied and 96 hours later a second microbiological sample was obtained (A2). The incidence of positive microbiological cultures and the number of cfus in stages A1 and A2 were compared statistically by the Wilcoxon test while the influence of the different treatments in intracanal infection was evaluated by Kruskal-Wallis test at 5% significance level (p<0.05). Large numbers of strict and facultative anaerobes, and viridans group streptococci were found in 100% of root canals of A1 samples. Among A2 samples, all treatments showed significant reduction of cfus and positive cultures (p<0.05), but only groups 3 and 4 showed 100% of root canals free of microorganisms. Rotary instrumentation plus NaOCl 5.25% associated with intracanal medication produced a drastic reduction or elimination of intracanal microbiota, whose performance was not influenced by the nature of the vehicle or the antiseptic added to the Ca(OH)(2) p.a.
本研究的目的是评估与用不同赋形剂和防腐剂配制的氢氧化钙糊剂联合使用的旋转器械的抗菌效果。在四只狗的72颗前磨牙根管中实验性诱导慢性根尖周病变。在严格的无菌条件下，在初始微生物取样（A1）后，使用Profile系统结合5.25%次氯酸钠对根管进行预备，并放置根管内药物。根据所使用的糊剂形成四个实验组：第1组-Calen（n=18），第2组-Calen+CPMC（n=20），第3组-Ca(OH)(2) p.a.+麻醉溶液（n=16），第4组-Ca(OH)(2) p.a.+2%葡萄糖酸氯己定（n=18）。21天后，取出糊剂；清空根管，96小时后获取第二个微生物样本（A2）。通过Wilcoxon检验对A1和A2阶段微生物培养阳性的发生率和菌落形成单位数量进行统计学比较，同时通过Kruskal-Wallis检验在5%显著性水平（p<0.05）下评估不同处理对根管内感染的影响。在A1样本的100%根管中发现了大量严格厌氧菌、兼性厌氧菌和草绿色链球菌。在A2样本中，所有处理均显示菌落形成单位和阳性培养物显著减少（p<0.05），但只有第3组和第4组显示100%的根管无微生物。旋转器械加5.25% NaOCl与根管内用药联合使用可使根管内微生物群大幅减少或消除，其效果不受赋形剂性质或添加到Ca(OH)(2) p.a.中的防腐剂的影响。
