Karabika E, Kallimanis A, Dados A, Pilidis G, Drainas C, Koukkou A I
Sector of Organic Chemistry and Biochemistry, Department of Chemistry, University of Ioannina, Ioannina, Greece.
Appl Biochem Biotechnol. 2009 Oct;159(1):155-67. doi: 10.1007/s12010-008-8463-1. Epub 2008 Dec 19.
A polycyclic aromatic hydrocarbon (PAH)-degrading bacterial strain Spyr1 was isolated from Greek creosote polluted soil by an enrichment method using pyrene as sole carbon and energy source. Spyr1 was identified as Mycobacterium sp. based on 16S rDNA analysis and it was capable of degrading pyrene, fluoranthene, fluorene, anthracene, and acenaphthene. The effect of entrapment in glass beads and alginate/starch mixtures on the survival and pyrene degradation ability of Spyr1 cells in liquid suspensions and soil microcosms was tested and compared with that of freely suspended cells. In general, free cells showed higher degradation of pyrene and other PAH than immobilized cells. However, immobilized cells could better tolerate PAH and they maintained their viability and PAH degradation capability for at least 1 year after storage at 4 degrees C. Entrapped cells in glass beads exhibited better pyrene biodegradation performance than alginate/starch entrapped cells in liquid suspensions and could be used effectively for at least ten repeated cycles. Alginate/starch entrapped cells exhibited better yields than glass beads entrapped cells for removing pyrene as well as mixtures of PAH in soil microcosms.
通过使用芘作为唯一碳源和能源的富集方法，从希腊杂酚油污染土壤中分离出一株多环芳烃（PAH）降解细菌菌株Spyr1。基于16S rDNA分析，Spyr1被鉴定为分枝杆菌属，它能够降解芘、荧蒽、芴、蒽和苊。测试了玻璃珠和海藻酸盐/淀粉混合物包埋对Spyr1细胞在液体悬浮液和土壤微观环境中的存活及芘降解能力的影响，并与自由悬浮细胞进行了比较。总体而言，游离细胞对芘和其他PAH的降解能力高于固定化细胞。然而，固定化细胞对PAH的耐受性更好，并且在4℃储存至少1年后仍能保持其活力和PAH降解能力。玻璃珠包埋的细胞在液体悬浮液中比海藻酸盐/淀粉包埋的细胞表现出更好的芘生物降解性能，并且可以有效地用于至少十个重复循环。在土壤微观环境中，海藻酸盐/淀粉包埋的细胞在去除芘以及PAH混合物方面比玻璃珠包埋的细胞表现出更高的产量。
