Collado Maria Carmen, Donat Ester, Ribes-Koninckx Carmen, Calabuig Miguel, Sanz Yolanda
Microbial Ecophysiology and Nutrition Group Institute of Agrochemistry and Food Technology, Spanish National Research Council (CSIC), Burjassot, Valencia, Spain.
BMC Microbiol. 2008 Dec 22;8:232. doi: 10.1186/1471-2180-8-232.
Gut bifidobacteria are believed to influence immune-related diseases. The objective of this study was to assess the possible relationships between the gut bifidobacteria composition and coeliac disease (CD) in children. A total of 48 faecal samples (30 and 18 samples from active and no active CD patients, respectively) and 33 duodenal biopsy specimens of CD patients (25 and 8 samples from active and non-active CD patients, respectively) were analysed. Samples (30 faecal samples and 8 biopsies) from a control age-matched group of children were also included for comparative purposes. Gut Bifidobacterium genus and species were analyzed by real-time PCR.
Active and non-active CD patients showed lower numbers of total Bifidobacterium and B. longum species in faeces and duodenal biopsies than controls, and these differences were particularly remarkable between active CD patients and controls. B. catenulatum prevalence was higher in biopsies of controls than in those of active and non-active CD patients, whereas B. dentium prevalence was higher in faeces of non-active CD patients than in controls. Correlations between levels of Bifidobacterium and B. longum species in faecal and biopsy samples were detected in both CD patients and controls.
Reductions in total Bifidobacterium and B. longum populations were associated with both active and non-active CD when compared to controls. These bacterial groups could constitute novel targets for adjuvant dietary therapies although the confirmation of this hypothesis would require further investigations.
肠道双歧杆菌被认为会影响免疫相关疾病。本研究的目的是评估儿童肠道双歧杆菌组成与乳糜泻（CD）之间的可能关系。共分析了48份粪便样本（分别来自活动期和非活动期CD患者的30份和18份样本）以及33份CD患者的十二指肠活检标本（分别来自活动期和非活动期CD患者的25份和8份样本）。还纳入了一组年龄匹配的对照儿童的样本（30份粪便样本和8份活检样本）用于比较。通过实时PCR分析肠道双歧杆菌属和种。
活动期和非活动期CD患者粪便和十二指肠活检标本中的双歧杆菌总数和长双歧杆菌数量均低于对照组，且这些差异在活动期CD患者与对照组之间尤为显著。链状双歧杆菌在对照组活检标本中的患病率高于活动期和非活动期CD患者，而龋齿双歧杆菌在非活动期CD患者粪便中的患病率高于对照组。在CD患者和对照组中均检测到粪便和活检样本中双歧杆菌和长双歧杆菌水平之间的相关性。
与对照组相比，双歧杆菌总数和长双歧杆菌数量的减少与活动期和非活动期CD均相关。这些细菌群体可能构成辅助饮食疗法的新靶点，不过这一假设的证实还需要进一步研究。
