Hedi Abdeljabbar, Fardeau Marie-Laure, Sadfi Najla, Boudabous Abdellatif, Ollivier Bernard, Cayol Jean-Luc
Laboratoire Microorganismes et Biomolécules Actives, Faculté des Sciences de Tunis, Université de Tunis El Manar, 2092 Tunis, Tunisia.
Extremophiles. 2009 Mar;13(2):313-9. doi: 10.1007/s00792-008-0218-y. Epub 2008 Dec 25.
A new halophilic anaerobe was isolated from the hypersaline surface sediments of El-Djerid Chott, Tunisia. The isolate, designated as strain 6SANG, grew at NaCl concentrations ranging from 14 to 30%, with an optimum at 20-22%. Strain 6SANG was a non-spore-forming, non-motile, rod-shaped bacterium, appearing singly, in pairs, or occasionally as long chains (0.7-1 x 4-13 microm) and showed a Gram-negative-like cell wall pattern. It grew optimally at pH values between 7.2 and 7.4, but had a very broad pH range for growth (5.9-8.4). Optimum temperature for growth was 42 degrees C (range 30-50 degrees C). Strain 6SANG required yeast extract for growth on sugars. Glucose, sucrose, galactose, mannose, maltose, cellobiose, pyruvate, and starch were fermented. The end products from glucose fermentation were acetate, butyrate, lactate, H(2), and CO(2). The G + C ratio of the DNA was 34.3 mol%. Strain 6SANG exhibited 16S rRNA gene sequence similarity values of 91-92% with members of the genus Halobacteroides, H. halobius being its closest phylogenetic relative. Based on phenotypic and phylogenetic characteristics, we propose that this bacterium be classified as a novel species of a novel genus, Halanaerobaculum tunisiense gen. nov., sp. nov. The type strain is 6SANG(T) (=DSM 19997(T)=JCM 15060(T)).
从突尼斯杰里德盐沼的高盐表层沉积物中分离出一种新的嗜盐厌氧菌。该分离菌株被命名为6SANG菌株，在氯化钠浓度为14%至30%的条件下生长，最适浓度为20 - 22%。6SANG菌株是一种不形成芽孢、无运动性的杆状细菌，单个、成对或偶尔呈长链状出现（0.7 - 1×4 - 13微米），呈现革兰氏阴性菌样细胞壁模式。它在pH值7.2至7.4之间生长最佳，但生长的pH范围很宽（5.9 - 8.4）。生长的最适温度为42℃（范围30 - 50℃）。6SANG菌株在利用糖类生长时需要酵母提取物。葡萄糖、蔗糖、半乳糖、甘露糖、麦芽糖、纤维二糖、丙酮酸和淀粉均可被发酵。葡萄糖发酵的终产物为乙酸盐、丁酸盐、乳酸盐、H₂和CO₂。DNA的G + C含量为34.3摩尔%。6SANG菌株与嗜盐杆菌属成员的16S rRNA基因序列相似性值为91 - 92%，嗜盐嗜盐杆菌是其最接近的系统发育亲缘关系。基于表型和系统发育特征，我们建议将该细菌归类为一个新属的新物种，即突尼斯嗜盐厌氧杆菌属新属，新种。模式菌株为6SANG(T)（=DSM 19997(T)=JCM 15060(T)）。
