Department of Chemistry and Biochemistry, College of Science and Engineering, University of Minnesota Duluth, 1039 University Drive, Duluth, Minnesota 55812, USA.
J Nat Prod. 2009 Feb 27;72(2):319-23. doi: 10.1021/np8005343.
Two new sulfated sesterterpene alkaloids, 19-oxofasciospongine A (3) and fasciospongine C (4), and a new sesterterpene sulfate, 25-hydroxyhalisulfate 9 (5), along with two known sesterterpene sulfates, halisulfates 7 (6) and 9 (7), were isolated from an organic extract of the marine sponge Fasciospongia sp. The structures of the new compounds were established on the basis of one- and two-dimensional NMR spectroscopic studies as well as by HRESIMS analysis. Compounds 1-7 exhibited inhibitory activity against Streptomyces 85E in the hyphae-formation inhibition assay. Compounds 1, 2, and 4-7 were not cytotoxic when tested at 20 microg/mL with MCF-7, LNCaP, and LU cell lines. Only compound 3 demonstrated a moderate cytotoxic effect on the MCF-7 (IC(50) 13.4 microM), LNCaP (IC(50) 21.8 microM), and LU-1 cells (IC(50) 5.0 microM), respectively.
从海洋海绵 Fasciospongia sp. 的有机提取物中分离得到两个新的磺化甾体生物碱，19-氧代 fasciospongine A (3) 和 fasciospongine C (4)，以及一个新的甾体硫酸盐 25-羟基 halisulfate 9 (5)，以及两个已知的甾体硫酸盐 halisulfates 7 (6) 和 9 (7)。新化合物的结构是基于一维和二维 NMR 光谱研究以及 HRESIMS 分析确定的。化合物 1-7 在菌丝形成抑制试验中对链霉菌 85E 表现出抑制活性。当用 MCF-7、LNCaP 和 LU 细胞系在 20 μg/mL 下测试时，化合物 1、2 和 4-7 均无细胞毒性。只有化合物 3 对 MCF-7 (IC50 13.4 μM)、LNCaP (IC50 21.8 μM) 和 LU-1 细胞 (IC50 5.0 μM) 分别表现出中等的细胞毒性作用。
