Filaire Edith, Dreux B, Massart A, Nourrit B, Rama L M, Teixeira A
Laboratoire AMAPP, UFRSTAPS-2, Allée du Château, Orléans Cedex, France.
Eur J Appl Physiol. 2009 May;106(1):71-7. doi: 10.1007/s00421-009-0991-z. Epub 2009 Feb 4.
The aim of this study was to (1) examine the presence of stress on professors when they teach in front of 200 students and analyse objectively such stress using biomarkers such as salivary cortisol, chromogranin A (CgA) and alpha-amylase (AA) (2) investigate whether sex affects the reactivity of salivary alpha-amylase (sAA) and cortisol concentrations and the interaction of both hormonal systems. Fifty-two participants (26 women and 26 men) collected nine unstimulated saliva samples on 2 days, (one working day, and one resting day). Cortisol concentrations and AA activity measured on the teaching day were significantly higher than those noted on the resting values. No differences between the resting day and the teaching day were reported for CgA. Our results showed a cortisol response to teaching, which was characterized by an anticipatory rise. The AA level was significantly increased after the end of the lecture, and returned to the pre-lecture level 30 min after the end of the lecture. The awakening cortisol response noted on the teaching day was significantly higher in females than in males. No baseline sex differences in sAA and CgA were observed and men and women seem to have a comparable reactivity in sAA, CgA and cortisol levels on lecture stress. The mechanisms that leads to modify activity of salivary AA and CgA due to stress is not entirely understood and further research is needed to elucidate them.
（1）检测教授在200名学生面前授课时的压力状况，并使用唾液皮质醇、嗜铬粒蛋白A（CgA）和α-淀粉酶（AA）等生物标志物对这种压力进行客观分析；（2）研究性别是否会影响唾液α-淀粉酶（sAA）的反应性和皮质醇浓度以及这两种激素系统的相互作用。52名参与者（26名女性和26名男性）在两天内（一个工作日和一个休息日）采集了9份非刺激性唾液样本。授课日测得的皮质醇浓度和AA活性显著高于休息日的值。CgA在休息日和授课日之间未报告有差异。我们的结果显示了对授课的皮质醇反应，其特征是预期性升高。讲座结束后AA水平显著升高，并在讲座结束后30分钟恢复到讲座前水平。授课日观察到的觉醒皮质醇反应在女性中显著高于男性。未观察到sAA和CgA的基线性别差异，男性和女性在讲座压力下sAA、CgA和皮质醇水平的反应性似乎相当。由于压力导致唾液AA和CgA活性改变的机制尚未完全了解，需要进一步研究以阐明它们。
