The Key Laboratory for Microbial Resources of the Ministry of Education, and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, PR China.
Quality and Standards Institute of Agricultural Products, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang 310021, PR China.
Int J Syst Evol Microbiol. 2012 Apr;62(Pt 4):764-769. doi: 10.1099/ijs.0.030361-0. Epub 2011 May 13.
A novel actinomycete strain, designated CW 59T, was isolated from a polluted forest soil sample in Anhui Province, China. Cells were strictly aerobic, non-motile, bent rods. The strain grew optimally at 30-37 °C and pH 6.0-8.0. The major fatty acids were ai-C15:0 (34.7 %), i-C15:0 (11.6 %) and ai-C17:0 (14.9 %); the predominant respiratory quinone was MK-9(H2), with MK-8(H2) present as a minor component. The polar lipid composition of strain CW 59T consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two glycolipids and phosphatidylmonomethylethanolamine (minor component). The whole-cell sugars contained galactose, mannose, ribose and glucose; the major amino acids of the cell-wall were lysine, alanine and glutamic acid. The genomic DNA G+C content was 66.9 mol%. Phylogenetic analysis showed that CW 59T belonged to the genus Sinomonas and grouped with members of the species Sinomonas atrocyanea, Sinomonas flava, Arthrobacter echigonensis and Arthrobacter albidus. 16S rRNA gene sequence similarities of CW 59T to S. atrocyanea DSM 20127T, S. flava CW 108T, A. echigonensis LC10T and A. albidus LC13T were 99.5, 99.3, 98.2 and 98.0 %, respectively. DNA-DNA hybridization of the isolate showed relatedness values of 58.3 % (DSM 20127T), 41.8 % (CW 108T), 21.6 % (LC10T) and 25.5 % (LC13T) with its four closest neighbours. The taxonomic relationships of strains LC10T and LC13T with the genus Sinomonas were further clarified by means of a direct experimental comparison; results showed that strains LC10T and LC13T showed the same major fatty acid, polar lipid, cell-wall amino acid, whole-cell sugar and respiratory quinone compositions as members of the genus Sinomonas. Based on phenotypic, chemotaxonomic and phylogenetic analysis, it is proposed that: strain CW 59T represents a novel species of the genus Sinomonas, Sinomonas soli sp. nov., with CW 59T (=CCTCC AB 207193T=KCTC 19389T) as the type strain; and the type strains of A. echigonensis and A. albidus should be reclassified as Sinomonas echigonensis comb. nov. and Sinomonas albida comb. nov., respectively. An emended description of the genus Sinomonas is given.
一株新的放线菌菌株，命名为 CW 59T，从中国安徽省受污染的森林土壤样本中分离得到。细胞严格需氧，无动力，弯曲的棒状。该菌株在 30-37°C 和 pH 6.0-8.0 下生长最佳。主要脂肪酸为 ai-C15:0（34.7%）、i-C15:0（11.6%）和 ai-C17:0（14.9%）；主要呼吸醌为 MK-9（H2），MK-8（H2）为次要成分。菌株 CW 59T 的极性脂组成包括双磷脂酰甘油、磷脂酰甘油、磷脂酰肌醇、两种糖脂和磷脂单甲醚乙醇胺（次要成分）。全细胞糖含有半乳糖、甘露糖、核糖和葡萄糖；细胞壁的主要氨基酸为赖氨酸、丙氨酸和谷氨酸。基因组 DNA G+C 含量为 66.9 mol%。系统发育分析表明，CW 59T 属于 Sinomonas 属，与 Sinomonas atrocyanea、Sinomonas flava、Arthrobacter echigonensis 和 Arthrobacter albidus 中的成员聚为一类。CW 59T 与 S. atrocyanea DSM 20127T、S. flava CW 108T、A. echigonensis LC10T 和 A. albidus LC13T 的 16S rRNA 基因序列相似性分别为 99.5%、99.3%、98.2%和 98.0%。与四个最接近的近缘种的 DNA-DNA 杂交显示，该分离株与 DSM 20127T 的相关性值为 58.3%，与 CW 108T 的相关性值为 41.8%，与 LC10T 的相关性值为 21.6%，与 LC13T 的相关性值为 25.5%。通过直接实验比较进一步阐明了 LC10T 和 LC13T 与 Sinomonas 属的分类关系；结果表明，LC10T 和 LC13T 具有相同的主要脂肪酸、极性脂、细胞壁氨基酸、全细胞糖和呼吸醌组成，与 Sinomonas 属的成员相同。基于表型、化学分类和系统发育分析，建议：菌株 CW 59T 代表 Sinomonas 属的一个新种，即 Sinomonas soli sp. nov.，CW 59T（=CCTCC AB 207193T=KCTC 19389T）为模式菌株；A. echigonensis 和 A. albidus 的模式菌株应分别重新分类为 Sinomonas echigonensis comb. nov.和 Sinomonas albida comb. nov.。对 Sinomonas 属进行了修订描述。
