Jin Yu, Cheng Wei-xia, Yang Xue-mei, Jin Miao, Zhang Qing, Xu Zi-qian, Yu Jie-mei, Zhu Lin, Yang Su-hua, Liu Na, Cui Shu-xian, Fang Zhao-yin, Duan Zhao-jun
Department of Pediatrics, The First Hospital of Lanzhou University, China.
J Clin Virol. 2009 Mar;44(3):238-41. doi: 10.1016/j.jcv.2008.12.010. Epub 2009 Feb 6.
Gastroenteritis is a major cause of childhood morbidity and mortality worldwide. Rotavirus, human caliciviruses (HucV), adenovirus, and astrovirus are recognized as common etiologies of acute gastroenteritis.
To use antigen detection and molecular methods to determine the viral etiology of childhood diarrhea in Lanzhou, China, 2005-2007.
544 stool specimens were collected from children hospitalized with diarrhea. ELISA, RT-PCR, or PCR were used to detect viruses commonly causing diarrhea.
Group A rotavirus, norovirus, sapovirus, astrovirus, and adenovirus, were detected in 54.0%, 9.2%, 1.1%, 3.3%, and 4.4%, respectively. No group B or group C rotaviruses were detected. The relative contribution of these viruses changed greatly over 2 years. The percentage of rotavirus and adenovirus dropped from 61.2% and 5.4% to 47.6% and 1.4%, whereas HucV increased from 5.0% to 15.0%. G1 and P[8] were the predominant rotavirus strains, and P[6] was detected for the first time in this area. The predominant norovirus strain changed from GII3 to GII4, and the subtypes of GII4 changed from the Hunter strain to the variant 2006b strain.
The distribution of viruses and genotypes of individual viruses causing gastroenteritis in Lanzhou, China changed greatly during 2005-2007.
在全球范围内，胃肠炎是儿童发病和死亡的主要原因。轮状病毒、人杯状病毒（HucV）、腺病毒和星状病毒被认为是急性胃肠炎的常见病因。
运用抗原检测和分子方法确定2005 - 2007年中国兰州儿童腹泻的病毒病因。
收集544例因腹泻住院儿童的粪便标本。采用酶联免疫吸附测定（ELISA）、逆转录聚合酶链反应（RT-PCR）或聚合酶链反应（PCR）检测常见的致腹泻病毒。
分别检测到A组轮状病毒、诺如病毒、札如病毒、星状病毒和腺病毒，阳性率分别为54.0%、9.2%、1.1%、3.3%和4.4%。未检测到B组或C组轮状病毒。这些病毒的相对构成在2年中发生了很大变化。轮状病毒和腺病毒的百分比从61.2%和5.4%降至47.6%和1.4%，而人杯状病毒从5.0%增至15.0%。G1和P[8]是主要的轮状病毒株，P[6]首次在该地区被检测到。主要的诺如病毒株从GII3变为GII4，GII4的亚型从Hunter株变为2006b变异株。
2005 - 2007年期间，中国兰州引起胃肠炎的病毒及其各病毒基因型的分布发生了很大变化。
