Kao Erl-Shyh, Hsu Jeng-Dong, Wang Chau-Jong, Yang Su-Huei, Cheng Su-Ya, Lee Huei-Jane
Department of Beauty Science, College of Humanity, Chienkuo Technology University, Changhua City, Taiwan.
Biosci Biotechnol Biochem. 2009 Feb;73(2):385-90. doi: 10.1271/bbb.80639. Epub 2009 Feb 7.
Oxidative stress and inflammation are related to several chronic diseases including cancer and atherosclerosis. Hibiscus sabdariffa Linnaeus has been found to possess antioxidant effects. In this study, polyphenols extracted from Hibiscus sabdariffa L. (HPE) were used to detect anti-inflammatory effects on nitrite and prostaglandin E(2) (PGE(2)) in lipopolysaccharide (LPS) treated RAW264.7 cells. Sequentially, an animal model examination was performed to confirm the effects of HPE on LPS-induced hepatic inflammation. The results showed that HPE reduced 94.6% of xanthine oxidase activity in vitro, and decreased nitrite and PGE(2) secretions in LPS-induced cells. In LPS-treated rats, HPE significantly decreased the serum levels of alanine and aspartate aminotransferase. In the liver, lipid peroxidation and liver lesions decreased, and catalase activity and glutathione increased. The study also revealed that down-regulation of cyclooxygenase-2 (COX-2), p-c-Jun N-terminal kinase (p-JNK) and p-P38 might have been involved. In sum, this study found an anti-inflammatory potency of HPE both in vitro and in vivo.
氧化应激和炎症与包括癌症和动脉粥样硬化在内的多种慢性疾病相关。已发现玫瑰茄具有抗氧化作用。在本研究中，从玫瑰茄中提取的多酚（HPE）用于检测其对脂多糖（LPS）处理的RAW264.7细胞中亚硝酸盐和前列腺素E2（PGE2）的抗炎作用。随后，进行动物模型实验以证实HPE对LPS诱导的肝脏炎症的影响。结果表明，HPE在体外降低了94.6%的黄嘌呤氧化酶活性，并减少了LPS诱导细胞中亚硝酸盐和PGE2的分泌。在LPS处理的大鼠中，HPE显著降低了血清丙氨酸和天冬氨酸转氨酶水平。在肝脏中，脂质过氧化和肝脏损伤减少，过氧化氢酶活性和谷胱甘肽增加。该研究还表明，环氧合酶-2（COX-2）、磷酸化c-Jun氨基末端激酶（p-JNK）和磷酸化P38的下调可能与之有关。总之，本研究发现HPE在体外和体内均具有抗炎效力。
