一段人类Y染色体DNA片段的进化与序列分析

Evolution and sequence analysis of a human Y-chromosomal DNA fragment.

作者信息

Whisenant E C, Rasheed B K, Ostrer H, Bhatnagar Y M

机构信息

Department of Structural & Cellular Biology, University of South Alabama, College of Medicine, Mobile 36688.

出版信息

J Mol Evol. 1991 Aug;33(2):133-41. doi: 10.1007/BF02193627.

DOI:10.1007/BF02193627

PMID:1920449

Abstract

A Y-chromosomal DNA fragment has been isolated from a human Y-Charon 21A recombinant library. Evolutionary analysis of 1F5 indicates that the size and sequence of this fragment have been conserved in higher primates. Deletion mapping and in situ hybridization analysis have localized 1F5 to the middle euchromatic portion of the long arm of the human Y chromosome at Yq11.2. Sequence analysis revealed the presence of an atypical Alu element and two regions rich in polypyrimidine-polypurine residues.

摘要

从人Y-Charon 21A重组文库中分离出一个Y染色体DNA片段。对1F5的进化分析表明，该片段的大小和序列在高等灵长类动物中保守。缺失作图和原位杂交分析已将1F5定位到人类Y染色体长臂的中部常染色质部分，位于Yq11.2。序列分析显示存在一个非典型Alu元件和两个富含聚嘧啶-聚嘌呤残基的区域。