Exercise is associated with elevated proinflammatory cytokines in human milk.

OBJECTIVES

To explore relationships between self-reported exercise in postpartum women and concentrations of cytokines and secretory immunoglobulin A in their milk.

METHOD

Fifty-eight frozen, unthawed aliquots of human hindmilk were available for analysis from a previous larger study on the influence of lactation on postpartum stress and immunity. The samples were early-morning, hand-expressed, hindmilk that had been collected between 4 and 6 weeks. Milk cytokines were analyzed by a multiplex assay of 20 cytokines, chemokines, and growth factors. Milk secretory immunoglobulin A was analyzed by enzyme-linked immunosorbent assay. Exercise data were extracted from a demographic questionnaire that was used in the original study and approximate metabolic-equivalent tasks assigned to the exercise levels reported. Based on reported frequency of exercise at a particular metabolic-equivalent task, caloric expenditures were calculated for each mother.

RESULTS

With increasing metabolic-equivalent tasks, and thus caloric expenditures, proinflammatory cytokines increased in mothers' milk. Secretory immunoglobulin A concentrations were not affected by mother's exercise.

CONCLUSIONS

There are several possible interpretations for these results. These data are preliminary, and a larger, longitudinal study with a more structured exercise instrument will clarify if recommendations should be made about heavy exercise in the early postpartum months.