Kawano Mitsuko, Han Junkyu, Kchouk Mohamed Elyes, Isoda Hiroko
Alliance for Research on North Africa, Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8572, Japan.
J Nat Med. 2009 Jul;63(3):335-9. doi: 10.1007/s11418-009-0324-x. Epub 2009 Mar 10.
Hair growth problems can affect human physical and mental health and are of particular relevance during the aging process. In an effort to resolve such problems, we attempted to find plants having hair growth regulation activity and thus collected plant extracts from Tunisia for bioprospecting purposes. Among them, we investigated the Erica multiflora extract to evaluate the hair growth promotion activity by using the MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide) assay and cell cycle assay on human dermal papilla cells in vitro and an administration assay on mouse dorsal skin in vivo. The results showed that the Erica multiflora extract promotes dermal papilla cell growth and cell cycle with high activity, and induced hair growth in vivo by induction of anagen phase from telogen phase.
毛发生长问题会影响人类身心健康，在衰老过程中尤为突出。为了解决这些问题，我们试图寻找具有毛发生长调节活性的植物，因此收集了突尼斯的植物提取物用于生物勘探。其中，我们研究了多花欧石楠提取物，通过体外对人毛囊乳头细胞进行MTT（3-(4,5-二甲基-2-噻唑基)-2,5-二苯基-2H-四唑溴盐）测定和细胞周期测定，以及体内对小鼠背部皮肤进行给药测定，来评估其促进毛发生长的活性。结果表明，多花欧石楠提取物具有高活性地促进毛囊乳头细胞生长和细胞周期，并通过将休止期诱导为生长期而促进体内毛发生长。
