Arbustini E, Grasso M, Diegoli M, Bramerio M, Foglieni A S, Albertario M, Martinelli L, Gavazzi A, Goggi C, Campana C
Department of Pathology, Università di Pavia, Italy.
Am J Pathol. 1991 Oct;139(4):709-15.
The authors performed an immunohistochemical study on expression of tumor necrosis factor alpha (TNF alpha) in endomyocardial biopsies from human cardiac allografts. TNF alpha immunoreactivity was found in 45% biopsies with mild acute rejection, in 83% biopsies with focal moderate rejection, in 80% biopsies with diffuse moderate rejection. Biopsies with absent rejection did not show immunoreactive cells. In mild rejection, positive cells were few and scanty monocytes and macrophages (MAC-387 and LN5 positive cells) and T lymphocytes (UCHL-1/CD45 RO positive cells) (up to 20% of all infiltrating cells). Expression of major histocompatibility complex (MHC) class II antigens on infiltrating and endothelial cells occurred earlier and independent of TNF alpha reactivity. Number of immunoreactive cells increased in moderate rejection (up to 50%). Immunoreactivity was also present in nonpigmented macrophages in part of the biopsies with resolving rejection (45%). The authors conclude that TNF alpha is expressed in acute cardiac rejection by immunologically activated inflammatory cells. Immunoreactive cells increase in number with increasing severity of the reaction.
作者对人类心脏移植内膜心肌活检组织中肿瘤坏死因子α（TNFα）的表达进行了免疫组织化学研究。在45%的轻度急性排斥活检组织、83%的局灶性中度排斥活检组织、80%的弥漫性中度排斥活检组织中发现了TNFα免疫反应性。无排斥反应的活检组织未显示免疫反应性细胞。在轻度排斥反应中，阳性细胞较少，有少量单核细胞和巨噬细胞（MAC - 387和LN5阳性细胞）以及T淋巴细胞（UCHL - 1/CD45 RO阳性细胞）（占所有浸润细胞的20%）。主要组织相容性复合体（MHC）II类抗原在浸润细胞和内皮细胞上的表达出现得更早，且与TNFα反应性无关。在中度排斥反应中，免疫反应性细胞数量增加（高达50%）。在部分正在消退的排斥反应活检组织（45%）中，无色素巨噬细胞也存在免疫反应性。作者得出结论，TNFα在急性心脏排斥反应中由免疫激活的炎症细胞表达。免疫反应性细胞数量随着反应严重程度的增加而增加。
