Bell Julia A, Kopper Jamie J, Turnbull Judy A, Barbu Nicholas I, Murphy Alice J, Mansfield Linda S
Comparative Enteric Diseases Laboratory, National Food Safety and Toxicology Center, Michigan State University, 181 Food Safety Building, East Lansing, MI 48824, USA.
Interdiscip Perspect Infect Dis. 2008;2008:149694. doi: 10.1155/2008/149694. Epub 2009 Jan 13.
We used terminal restriction fragment polymorphism (T-RFLP) analysis to assess (1) stability of the fecal microbiota in dogs living in environments characterized by varying degrees of exposure to factors that might alter the microbiota and (2) changes in the microbiota associated with acute episodes of diarrhea. Results showed that the healthy canine GI tract harbors potential enteric pathogens. Dogs living in an environment providing minimal exposure to factors that might alter the microbiota had similar microbiotas; the microbiotas of dogs kept in more variable environments were more variable. Substantial changes in the microbiota occurred during diarrheic episodes, including increased levels of Clostridium perfringens, Enterococcus faecalis, and Enterococcus faecium. When diet and medications of a dog having a previously stable microbiota were changed repeatedly, the microbiota also changed repeatedly. Temporal trend analysis showed directional changes in the microbiota after perturbation, a return to the starting condition, and then fluctuating changes over time.
我们使用末端限制性片段长度多态性（T-RFLP）分析来评估：（1）生活在不同程度接触可能改变微生物群因素环境中的犬类粪便微生物群的稳定性；（2）与腹泻急性发作相关的微生物群变化。结果表明，健康犬的胃肠道中存在潜在的肠道病原体。生活在极少接触可能改变微生物群因素环境中的犬类具有相似的微生物群；生活在变化更多环境中的犬类微生物群则更具变异性。腹泻发作期间微生物群发生了显著变化，包括产气荚膜梭菌、粪肠球菌和屎肠球菌水平升高。当先前微生物群稳定的犬类饮食和药物反复改变时，微生物群也会反复变化。时间趋势分析表明，扰动后微生物群发生定向变化，恢复到起始状态，然后随时间波动变化。
