Persson Fredrik, Utko Pawel, Reisner Walter, Larsen Niels B, Kristensen Anders
Department of Micro- and Nanotechnology, Technical University of Denmark, DTU Nanotech, Kongens Lyngby, Denmark.
Nano Lett. 2009 Apr;9(4):1382-5. doi: 10.1021/nl803030e.
We demonstrate a confinement spectroscopy technique capable of probing small conformational changes of unanchored single DNA molecules in a manner analogous to force spectroscopy, in the regime corresponding to femtonewton forces. In contrast to force spectroscopy, various structural forms of DNA can easily be probed, as indicated by experiments on linear and circular DNA. The extension of circular DNA is found to scale according to the de Gennes exponent, unlike for linear DNA.
我们展示了一种限制光谱技术，该技术能够以类似于力谱的方式，在对应于飞牛级力的范围内探测未锚定的单个DNA分子的微小构象变化。与力谱不同的是，正如对线性和环状DNA的实验所示，DNA的各种结构形式都能很容易地被探测到。与线性DNA不同，发现环状DNA的伸长符合德热纳指数规律。
