Li Peng, Zhao Xuelong, Zhang Li, Ding Anwei
Key Laboratory for Modern Research of Traditional Chinese Medical Formulae of Jiangsu, Nanjing University of Traditional Chinese Medicine, Nanjing 210046 China.
Zhongguo Zhong Yao Za Zhi. 2009 Oct;34(19):2463-5.
To establish the analytical method of the HPLC fingerprint of Cortex Moutan Carbonisatum and offer the evidence for quality control and quality evaluation of carbonized Cortex Moutan accordingly.
Ten batches of Cortex Moutan Carbonisatum were measured by HPLC with Paeonol as a reference substance and the HPLC analysis was performed on a Hedera ODS-3 Chromatographic Column (4.6 mm x 150 mm, 5 microm), with methanol to Acetonitrile (1:1) and 0.5% trifluoroacetic acid solution as mobile phase in gradient mode and the detection wavelength was at 258 nm.
The common mode of the HPLC fingerprints were set up. There were 11 common peaks in the fingerprint of ten samples, and the similar degrees to the ten batches were between 0.830-0.991.
The method is simple, accurate and have a good reaptability. And the quality of Cortex Moutan Carbonisatum can be controlled effectively by the HPLC fingerprint.
建立牡丹皮炭的高效液相色谱指纹图谱分析方法，为牡丹皮炭的质量控制和质量评价提供依据。
以丹皮酚为对照品，采用高效液相色谱法测定10批牡丹皮炭，色谱柱为Hedera ODS - 3柱（4.6 mm×150 mm，5μm），流动相为甲醇 - 乙腈（1∶1）和0.5%三氟乙酸溶液，梯度洗脱，检测波长为258 nm。
建立了高效液相色谱指纹图谱的共有模式。10个样品指纹图谱中有11个共有峰，10批样品之间的相似度在0.830 - 0.991之间。
该方法简便、准确、重复性好，高效液相色谱指纹图谱可有效控制牡丹皮炭的质量。
