Urdaz-Rodríguez J H, Fosgate G T, Alleman A R, Rae D O, Donovan G A, Melendez P
Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL 32610, USA.
Trop Anim Health Prod. 2009 Oct;41(7):1439-48. doi: 10.1007/s11250-009-9332-9. Epub 2009 Apr 1.
A cross-sectional study was conducted to determine individual cow seroprevalence of Anaplasma marginale in adult lactating dairy cattle of Puerto Rico (PR) and to assess the associations of farm management factors on herd seroprevalence. Antibody activity against A. marginale was determined using the MSP-5 competitive enzyme-linked immunosorbent assay. Serum samples were obtained from 2,414 adult lactating dairy cattle from 76 randomly selected commercial dairy farms. Herd seroprevalence ranged from 3 to 100% with an overall individual cow seroprevalence for A. marginale of 27.4%. Factors associated with high herd seropositivity were pasture grazing as the main feed source (OR = 6.5, 95% CI = 1.2-34), observed monkeys on the premises (OR = 13, 95% CI = 1.2-138), use of 11% permethrin (OR = 17, 95% CI = 2.2-129), farmers who attended an acaricide certification program (OR = 0.18, 95% CI = 0.04-0.74), and lack of a fly control program (OR = 5.6, 95% CI = 1.3-24).
开展了一项横断面研究，以确定波多黎各成年泌乳奶牛中边缘无形体的个体牛血清阳性率，并评估农场管理因素与畜群血清阳性率之间的关联。使用MSP-5竞争酶联免疫吸附测定法测定针对边缘无形体的抗体活性。从76个随机选择的商业奶牛场的2414头成年泌乳奶牛中采集血清样本。畜群血清阳性率在3%至100%之间，边缘无形体的个体牛总体血清阳性率为27.4%。与高畜群血清阳性相关的因素包括以放牧为主要饲料来源（比值比=6.5，95%置信区间=1.2-34）、在农场观察到猴子（比值比=13，95%置信区间=1.2-138）、使用11%氯菊酯（比值比=17，95%置信区间=2.2-129）、参加杀螨剂认证计划的农民（比值比=0.18，95%置信区间=0.04-0.74）以及缺乏苍蝇控制计划（比值比=5.6，95%置信区间=1.3-24）。
