Institute of Chinese Pharmaceutical Sciences, Taichung 40402, Taiwan.
Evid Based Complement Alternat Med. 2011;2011:478246. doi: 10.1093/ecam/nep027. Epub 2010 Oct 25.
Hispolon, an active ingredient in the fungi Phellinus linteus was evaluated with analgesic and anti-inflammatory effects. Treatment of male ICR mice with hispolon (10 and 20 mg/kg) significantly inhibited the numbers of acetic acid-induced writhing response. Also, our result showed that hispolon (20 mg/kg) significantly inhibited the formalin-induced pain in the later phase (P<.01). In the anti-inflammatory test, hispolon (20 mg/kg) decreased the paw edema at the fourth and fifth hour after λ-carrageenin (Carr) administration, and increased the activities of superoxide dismutase (SOD), glutathione peroxidase (GPx) and glutathione reductase (GRx) in the liver tissue. We also demonstrated that hispolon significantly attenuated the malondialdehyde (MDA) level in the edema paw at the fifth hour after Carr injection. Hispolon (10 and 20 mg/kg) decreased the nitric oxide (NO) levels on both the edema paw and serum level at the fifth hour after Carr injection. Also, hispolon (10 and 20 mg/kg) diminished the serum TNF-α at the fifth hour after Carr injection. The anti-inflammatory mechanisms of hispolon might be related to the decrease in the level of MDA in the edema paw by increasing the activities of SOD, GPx and GRx in the liver. It probably exerts anti-inflammatory effects through the suppression of TNF-α and NO.
松杉灵芝中的活性成分云芝素有镇痛和抗炎作用。雄性 ICR 小鼠经云芝素（10 和 20mg/kg）处理后，明显抑制醋酸诱导的扭体反应次数。此外，我们的结果表明，云芝素（20mg/kg）显著抑制福尔马林诱导的后期疼痛（P<.01）。在抗炎试验中，云芝素（20mg/kg）在角叉菜胶（Carr）给药后第 4 和第 5 小时降低了爪肿胀，并增加了肝组织中超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPx）和谷胱甘肽还原酶（GRx）的活性。我们还表明，云芝素显著降低了 Carr 注射后第 5 小时肿胀爪中的丙二醛（MDA）水平。云芝素（10 和 20mg/kg）降低了 Carr 注射后第 5 小时肿胀爪和血清中一氧化氮（NO）的水平。此外，云芝素（10 和 20mg/kg）降低了 Carr 注射后第 5 小时血清中的 TNF-α水平。云芝素的抗炎机制可能与通过增加肝组织中 SOD、GPx 和 GRx 的活性降低肿胀爪中 MDA 的水平有关。它可能通过抑制 TNF-α和 NO 发挥抗炎作用。
