用乙烯亚胺灭活猪肠道病毒免疫的仔猪胃肠道内容物中的中和活性。
Neutralizing activity in the gastrointestinal contents of piglets vaccinated with an ethylenimine-inactivated porcine enterovirus.
作者信息
Hazlett D G, Derbyshire J B
出版信息
Can J Comp Med. 1977 Apr;41(2):160-5.
The T80 strain of porcine enterovirus was rapidly and completely inactiviated by ethylenimine in a reaction which appeared to follow first order kinetics. The virus was effectively concentrated 35- to 88-fold, with recovery rates of 23 t0 53%, by adsorption to the polyelectrolyte PE60. Multiple doses of adjuvanted, PE60-concentrated, ethylenimine-inactivated T80 virus given by both the oral and subcutaneous routes induced the appearance of significant levels of virus neutralizing activity in the gastrointestinal tract of piglets vaccinated at four weeks of age. This activity, found predominantly in large intestine, was present 14 days after administration of the first dose of vaccine and significant levels of activity were still detectable six weeks later. Titres of serum virus neutralizing activity were higher and more persistent than in piglets which received live or formaldehyde-inactivated T80 virus by the oral or intramuscular routes.
猪肠道病毒T80株在与乙烯亚胺的反应中迅速且完全失活，该反应似乎遵循一级动力学。通过吸附到聚电解质PE60上，病毒有效地浓缩了35至88倍，回收率为23%至53%。口服和皮下途径给予多剂量的佐剂化、PE60浓缩、乙烯亚胺灭活的T80病毒，在四周龄接种疫苗的仔猪胃肠道中诱导出显著水平的病毒中和活性。这种活性主要存在于大肠中，在首次接种疫苗后14天出现，六周后仍可检测到显著水平的活性。血清病毒中和活性的滴度比通过口服或肌肉途径接受活的或甲醛灭活的T80病毒的仔猪更高且更持久。
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