人鼻上皮细胞培养系统：对人干扰素反应的评估
Human nasal epithelial cell culture system: evaluation of response to human interferons.
作者信息
Harmon M W, Greenberg S B, Johnson P E, Couch R B
出版信息
Infect Immun. 1977 May;16(2):480-5. doi: 10.1128/iai.16.2.480-485.1977.
Abstract
This report describes the response of human nasal epithelial cells to exogenously applied human interferon (IF). To measure the response, a system developed to study human nasal epithelial cells in vitro was used. Nasal epithelial cells responded equally to both leukocyte and fibroblast IF, with development of resistance to virus replication that was time and dose dependent. Cells required contact with 1,000 U of IF (400 69/19 reference units) for 4 h to produce consistently a significant reduction in virus yield. This in vitro cell cultured system may be useful in predicting the in vivo response of human IF in respiratory viral infections.
摘要
本报告描述了人鼻上皮细胞对外源性应用的人干扰素（IF）的反应。为了测量该反应，使用了一个开发用于体外研究人鼻上皮细胞的系统。鼻上皮细胞对白细胞干扰素和成纤维细胞干扰素的反应相同，对病毒复制产生的抗性具有时间和剂量依赖性。细胞需要与1000 U的干扰素（400 69/19参考单位）接触4小时，才能持续显著降低病毒产量。这种体外细胞培养系统可能有助于预测人干扰素在呼吸道病毒感染中的体内反应。
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